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Journal of Fluorescence

, Volume 29, Issue 4, pp 827–835 | Cite as

Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers

  • Akram Najaran
  • Adeleh DivsalarEmail author
  • Ali Akbar Saboury
  • Nasim Hayati Roodbari
ORIGINAL ARTICLE

Abstract

Considering the importance of pharmacology and the influence of drugs on biological materials, the effects of a newly designed and synthesized platin complex (2,2′-Bipyridine-3,3′-dicarboxylic acid, oxalato Platinum(II), as an antitumor drug was tested on the structure of blood carrier protein of human serum albumin (HSA) using various spectroscopic techniques including UV-visible, fluorescence, and circular dichroism at 25 and 37 °C. Results of the fluorescence measurements revealed that adding the complex caused reduction in intrinsic fluorescence emission of HSA resulted from dynamic quenching of HSA. The number of binding sites and binding constants were calculated at both temperatures of 25 and 37 °C. In addition, in order to identify the complex’s binding site on HSA employing spectroscopy, the competitive studies were followed using warfarin, digitoxin and ibuprofen as site markers of Sudlow sites I, II and III. Competitive binding test results have shown that Pt(II) complex bind on the warfarin binding site (or Sudlow sites I) on HSA. Besides, a reduction in thermal stability for HSA was observed in the presence of the newly designed Pt(II) complex.

Keywords

Site markers HSA Sudlow sites Competitive studies Fluorescence quenching 

Notes

Acknowledgments

Authors thank the Research Council of Kharazmi University for financial support of this work.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Akram Najaran
    • 1
  • Adeleh Divsalar
    • 2
    Email author
  • Ali Akbar Saboury
    • 3
  • Nasim Hayati Roodbari
    • 1
  1. 1.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Cell & Molecular Sciences, Faculty of Biological SciencesKharazmi UniversityTehranIran
  3. 3.Institute of Biochemistry and BiophysicsUniversity of TehranTehranIran

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