Journal of Fluorescence

, Volume 29, Issue 1, pp 195–201 | Cite as

A Dicyanomethylene-4H-Pyran Based NIR Ratiometric Fluorescent Probe for Diazane and its Bioimaging

  • Gongchun Li
  • Yongxiang Liu
  • Xiaopeng Yang
  • Yong YeEmail author


A near-infrared ICT-based fluorescent probe LX was successfully obtained. LX which detection limit is low as 22.2 nm shows excellent selectivity and high sensitivity to diazane. LX can selectively detected diazane from other species over a wide pH (3–10) range. A obvious color change of solution from yellow to orange can be found, allowing the naked eye to detect. The sensing mechanism was reasonably detected by ESI-MS and DFT calculations. In addition, LX succeed in the visualization of diazane in living cells and the detection of diazane in water samples.


Fluorescent probe Diazane NIR ICT 



This work was financially supported by NSFC (No. 21572209) and Program for Innovative Research Team (in Science and Technology) in University of Henan Province (No. 17IRTSTHN002).

Supplementary material

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ESM 1 (DOC 1605 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key laboratory of Chemo/Biosensing and Detection, College of Chemistry and Chemical EngineeringXuchang UniversityXuchangChina
  2. 2.College of Chemistry and Molecular EngineeringZhengzhou UniversityZhengzhouChina

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