Synthesis and Studies of Fluorescein Based Derivatives for their Optical Properties, Urease Inhibition and Molecular Docking
- 17 Downloads
Herein, we design and synthesized new fluorescein based derivatives by insitu formation of fluorescein ester and further treated with corresponding hydrazide and amine to yield respective compounds i.e. FB1, FB2, FB3 and FB4. The spectral purity and characterization was done by using IR, NMR and Mass spectroscopies. The synthesized derivatives were examined for their photophysical properties by using variety of organic solvents and results were discussed in details. The structural diversity of synthesized compounds motivate us to evaluate these compounds for urease inhibition. The compound FB3 (IC50 = 0.0456 μM) shows 100 fold more active against Jack bean urease than standard drug thiourea (IC50 = 4.7455 μM). Other synthesized compounds showed potent activity. Free radical percentage scavenging assay further supported the capacity of compounds to urease inhibition. While, molecular docking simulations helps to examine the molecular interactions of active compounds FB1, FB2, FB3 and FB4 within the binding site of urease enzyme.
KeywordsSynthesis Fluorescein derivatives Optical properties Urease inhibitors Molecular docking
This work was supported by a grant from Mid-Career Researcher Program (NRF- 2016R1A2B4016552) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP).
- 7.Brush CK (1991) Fluorescein labelled phosphoramidites, US Patent 5,583,236Google Scholar
- 35.Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A (2005) (In) John M. Walker (ed): The proteomics protocols handbook, Humana Press. 571–607Google Scholar
- 42.Valeur B (2001) Molecular fluorescence: principles and applications. Wiley-VcH Verlag GmbH,Google Scholar
- 50.Channar PA, Saeed A, Albericio F, Larik FA, Abbas Q, Hassan M, Raza H, Seo SY (2017) Sulfonamide-linked ciprofloxacin, sulfadiazine and amantadine derivatives as a novel class of inhibitors of Jack bean urease; synthesis, kinetic mechanism and molecular docking. Molecules 22:1352. https://doi.org/10.3390/molecules22081352 CrossRefGoogle Scholar