3-Hydroxyphenylboronic Acid-Based Carbon Dot Sensors for Fructose Sensing
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The selective fluorescence sensing of fructose was achieved by fluorescence quenching of the emission of hydrothermal-synthesized carbon quantum dots prepared by 3-hydroxyphenylboronic acid. Quantification of fructose was possible in aqueous solutions with pH of 9 (Limit of Detection LOD and Limit of Quantification LOQ of 2.04 and 6.12 mM), by quenching of the emission at 376 nm and excitation ~380 nm with a linearity range of 0–150 mM. A Stern-Volmer constant (KSV) of 2.11 × 10−2 mM−1 was obtained, while a fluorescent quantum yield of 31% was calculated. The sensitivity of this assay towards fructose was confirmed by comparison with other sugars (such as glucose, sucrose and lactose). Finally, the validity of the proposed assays was further demonstrated by performing recovery assays in different matrixes.
KeywordsCarbon dots Boronic acids Fructose Sensing Fluorescence quenching
This work was made in the framework of the project Sustainable Advanced Materials (NORTE-01-00145-FEDER- 000028), funded by “Fundo Europeu de Desenvolvimento Regional (FEDER)”, through “Programa Operacional do Norte” (NORTE2020). Acknowledgment to project POCI- 01-0145-FEDER-006980, funded by FEDER through COMPETE2020, is also made. The Laboratory for Computational Modeling of Environmental Pollutants−Human Interactions (LACOMEPHI) is acknowledged. L.P. d.S. also acknowledges a post-doctoral grant funded by project Sustainable Advanced Materials (NORTE-01-00145-FEDER-000028). Projects PTDC/QEQ-QFI/0289/2014 and PTDC/QEQ-QAN/5955/ 2014 are also acknowledged. These projects are cofunded by FCT/MEC (PIDDAC) and by FEDER through “COMPETE− Programa Operacional Fatores de Competitividade” (COMPETE-POFC).
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