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Synergistic Effects of Volatiles from Host-Infested Plants on Host-Searching Behavior in the Parasitoid Wasp Lytopylus rufipes (Hymenoptera: Braconidae)

  • Chia-Ming Liu
  • Shigeru Matsuyama
  • Yooichi KainohEmail author
Article

Abstract

Herbivore-induced plant volatiles (HIPVs) are important cues for natural enemies to find their hosts. HIPVs are usually present as blends and the effects of combinations of individual components are less studied. Here, we investigated plant volatiles in a tritrophic system, comprising the parasitoid wasp Lytopylus rufipes Nees (Hymenoptera: Braconidae), the Oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae), and Japanese pear, Pyrus pyrifolia ‘Kosui’, so as to elucidate the effects of single components and blends on wasp behaviors. Bioassays in a four-arm olfactometer, using either shoots or their isolated volatiles collected on adsorbent, revealed that female wasps preferred volatiles from host-infested shoots over those from intact shoots. Analyses identified (Z)-3-hexenyl acetate (H), linalool (L), (E)-β-ocimene (O), (E)-3,8-dimethyl-1,4,7-nonatriene (D), and (E,E)-α-farnesene (F). Among them, only F was induced by infestation with G. molesta. When tested singly, only O and D elicited positive responses by L. rufipes. Binary blends of HO and DF elicited a positive response, but that of HD elicited a negative one, even though D alone elicited a positive response. Remarkably, wasps did not prefer either the ODF or HL blends, but showed a highest positive response to a quinary blend (HLODF). These results show that synergism among volatiles released from host-infested plants is necessary for eliciting high behavioral responses in L. rufipes, enabling L. rufipes to find its host efficiently.

Keywords

Lytopylus rufipes (E)-β-ocimene (E,E)-α-farnesene (E)-DMNT HIPV Plant volatiles 

Notes

Acknowledgements

We appreciate the kind help of the following researchers: Mr. Masao Kaneko (Nagano Fruit Tree Experiment Station) for the moth colony; Mr. Masataka Sasaki (Fukushima Plant Protection Association) for the wasp colony; Dr. Masahiro Arakawa and Mr. Kouki Yoshida (Fruit Tree Research Center, Fukushima Prefecture) for preparing the thinned apples for insect rearing; Prof. Junko Sugaya (Faculty of Life and Environmental Sciences, University of Tsukuba) for the pear trees and advice on their care; and Prof. DeMar Taylor for English editing.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaIbarakiJapan
  2. 2.Faculty of Life and Environmental SciencesUniversity of TsukubaIbarakiJapan

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