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Journal of Chemical Ecology

, Volume 44, Issue 10, pp 905–914 | Cite as

Particular Levels of Odors Released by Virgin Females Attract Conspecific Males of the Funnel-Web Spider Allagelena difficilis

  • Zhi-Wu Chen
  • Yi-Fan Zhao
  • Shi-Cong He
  • Ke-Ke Liu
  • Ji-He Liu
  • Yong-Hong Xiao
Article
  • 99 Downloads

Abstract

Female-released chemical signals are crucial clues for mate-searching males to locate and gain sexual receptivity of conspecific females. Abundant behavioral evidence indicates that female spiders release sex pheromones to guide mate-searching behavior of conspecific mature males. However, the chemical nature of spider pheromones is poorly understood. Females of the funnel-web spider, Allagelena difficilis, employ sit-and-wait tactics for mating. Field observations indicate that males leave their retreats to search for potential mates during the breeding season. Therefore, we investigated whether virgin females release a sex attractant to conspecific males and then explored the chemical nature of the female pheromone. Four fatty acids extracted from the female bodies (palmitic acid, linoleic acid, cis-vaccenic acid and stearic acid) constitute a multiple-component sex attractant to conspecific males in A. difficilis. Unexpectedly, mated females also produce the same fatty acids, but at trace levels. Two-choice experiments showed that males were significantly attracted by the blend of the four fatty acids in appropriate concentrations while avoiding the blend consisting of the same acids at very low concentrations, suggesting that mate-searching males are able to discriminate virgin females from mated females by the quantities of female-specific fatty acids in the funnel-web spider A. difficilis.

Keywords

Female pheromone Chemical communication Sexual attractant Cuticular lipids Allagelena difficilis Spider 

Notes

Acknowledgments

This work was funded by the Natural Sciences Foundation of China (NSFC 31560592, 31471963, 31772423) and the Science and Technology Foundation of Jiangxi Provincial Department of Education (GJJ160753). We thank Zeyuan Meng, Wenjun Xie (College of Life Sciences, Jinggangshan University, China) for collecting spiders with the authors.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life SciencesJinggangshan UniversityJi’anChina
  2. 2.School of Agricultural SciencesJiangxi Agricultural UniversityNanchangChina
  3. 3.College of Life SciencesHunan Normal UniversityChangshaChina

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