A New Cluster-Based MOF for Selective Gas Sorption and Treatment Effect on Acute glomerulonephritis by Reducing NF-κb Pathway Activation and Cytokines Release
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A novel Co(II)-containing metal–organic framework [Co3(OH)2(H2TCPP)2](DMF)3 (1) based on a linear trinuclear Co(II) cluster-based unit has been successfully prepared by using a nanosized tetracarboxylate ligand 2,3,5,6-tetrakis(4-carboxyphenyl)pyrazine (H4TCPP) as the organic building block under the solvothermal reaction. Gas adsorption studies reveal that the mixtures of C2H2/CH4 and C2H2/CO2 could be selectively separated by the obtained resulting solvent free 1 (denoted as 1a hereafter). To deep evaluate the protective function of complex for acute glomerulonephritis (AGN) disease in vitro, the following experiments were conducted. (a) Real time Reverse transcription-Polymerase Chain Reaction (RT-PCR) was carried out to determine the relative expression of Nuclear factor kappa beta (nf-κb) and Tumor Necrosis Factor-α (tnf-α) in the glomerulus cells after bacterial infection along with complex 1 treatment. (b) The enzyme linked immunosorbent assay (ELISA) was conducted to determine the IL-1β and IL-6 released by glomerular cells after complex 1 treatment. (c) The results obtained through molecular docking exhibited possible regulation mechanism on the binding affinity between compound 1 and target protein.
KeywordsMOF Co(II)-complex Porous framework C2H2/CO2 separation Glomerular cells Inflammatory response Molecular docking
- 16.X. Feng, H. L. Song, B. X. Ye, S. Z. Huo, S. Y. Xie, and J. Z. Guo (2014). Chin. J. Struc. Chem. 33, 897.Google Scholar