Biofabrication of AgNPs Nanoparticles and Their Loading with Sevoflurane, a Volatile Anesthetic Drug for Therapeutic Treatment of Focal Cerebral Ischemia-In-Vivo Studies in Rats
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The process of green reduction was used to prepare the colloidal mixture of silver nanoparticles (AgNPs) with the help of silver nitrate (AgNO3), which is used as a precursor along with the existence of Bischofia javanica leaf extract that plays both as a reducing as well as capping agent. An aesthetic drug which is volatile in nature and is used to form nano-drug complex (SF-AgNPs), Sevoflurane (SF) was used to functionalize the fabricated AgNPs. The interaction and loading of the drug with AgNPs is studied with the help of several analytical mechanisms such as dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and ultraviolet–visible (UV–Vis) absorption spectra. Several reflections in the XRD spectra and the shift observed in the SPR peak of UV–Vis is accredited to the loading of SF on the surface of AgNPs. When related with the bare NPs, the growth in the size of SF-loaded NPs confirms the loading of drug. Further investigation disclosed that the SF-AgNPs of 20 nm increased the fabrication of anti-inflammatory cytokines, along with the inhibition of ischemia–reperfusion induced microglias and astrocytes activation. In addition, the molecules of pro-apoptotic were down regulated and the expressions of anti-apoptotic proteins were up regulated in the post-ischemic brains. When examining the group treated with 15 nm AgNPs, these effects were noticed to be conflicting. These responses exposed that the neuroprotection of SF-AgNPs 20 nm was ascribable to its anti-apoptotic as well as anti-inflammatory effects. The present work can deliver a unique approach for the healing of cerebral ischemia–reperfusion injury. Owing to the above results, this work provided modernized understandings into the self-treatment of SF-AgNPs for cerebral ischemic stroke (IS).
KeywordsAgNPs Cerebral ischemia Sevoflurane
Authors are thankful to Tai’an City central hospital, Shandong province for providing platform to do this research.
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