In Vivo Toxicity Assessment of Laminarin Based Silver Nanoparticles from Turbinaria ornata in Adult Zebrafish (Danio rerio)
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Silver nanoparticles synthesized using laminarin from Turbinaria ornata were subjected to acute toxicity in adult zebrafish. LC50 concentration of L-AgNPs and C-AgNPs at 96 h was noted to be 25.22 and 19.93 mg/L. To find out the fundamental toxicity mechanisms of L-AgNPs and C-AgNPs, the half of LC50 concentration 12.61 and 9.96 mg/L was exposed to adult zebrafish for 14 days respectively. There are no cytological changes in L-AgNPs whereas cytological changes were observed in gills, brain and liver tissues of C-AgNPs. The results concluded a plausible symbol for oxidative stress in zebrafish. The activities of antioxidant enzyme superoxide dismutase, catalase and glutathione peroxidase were decreased significantly in the C-AgNPs exposed while L-AgNPs showed no significant difference when compared to control. ICP-OES analysis of gills, liver and brain demonstrated that L-AgNPs and C-AgNPs revelation for 14 days resulted in enhanced silver uptake compared to fish treated with control are discussed in detail.
KeywordsLaminarin Silver nanoparticles Toxicity Turbinaria ornata ICP-OES Zebrafish (Danio rerio)
The authors gratefully acknowledge Central Leather Research Institute–Centre for Analysis, Testing, Evaluation and Reporting Services, Adyar, Chennai for providing the instrumentation facility (ICP-OES).
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