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Journal of Cluster Science

, Volume 30, Issue 6, pp 1393–1402 | Cite as

Novel and Facile Synthesis of Sea Anemone Adhesive Protein-Coated ZnO Nanoparticles: Antioxidant, Antibiofilm, and Mosquito Larvicidal Activity Against Aedes aegypti

  • Muthukumar Abinaya
  • Ravichandran Rekha
  • Shanthini Sivakumar
  • Marimuthu Govindarajan
  • Naiyf S. Alharbi
  • Shine Kadaikunnan
  • Jamal M. Khaled
  • Ahmed S. Alobaidi
  • Mohammed N. Al-Anbr
  • Baskaralingam VaseeharanEmail author
Article
  • 100 Downloads

Abstract

Recently, marine bio-products have provided a new insight on biomaterials for pharmaceutical applications. Herein, the use of adhesive protein in the fabrication of bio-inspired nanomaterials noted to the possible realization of vital biomedical applications. This study illustrates the biosynthesize ZnO nanoparticles (ZnO NPs) using adhesive protein from sea anemone Stichodactyla haddoni (ShAp). ShAp-ZnO NPs were characterized via SDS-PAGE, UV–Visible, XRD, FTIR, TEM and SAED. The molecular weight of the adhesive protein was determined by SDS-PAGE to be between 36 and 42 kDa. The UV–Vis peak of ShAp-ZnO NPs was revealed at 210 nm while XRD exhibited the crystalline nature of ShAp-ZnO NPs. FTIR revealed that functional group of ShAp-ZnO NPs, which exhibited peaks at 3441.31–1073.36 cm−1. HR-TEM revealed that ShAp-ZnO NPs obtained structure were hexagonal with 10 nm diameter. The antioxidant properties of ShAp, zinc acetate, and ShAp-ZnO NPs were noted at 100 µg/mL. Further, microscopic analysis demonstrated that 50 µg/mL of ShAp, zinc acetate, and ShAp-ZnO NPs efficiently repressed the biofilm formation of both Gram(+) and Gram(−) bacteria. In addition, maximal larvicidal effects were noticed at 100 µg/mL of ShAp-ZnO NPs against the 3rd instar of Aedes aegypti. Overall, the ShAp-ZnO NPs could have entomological and pharmaceutical applications.

Keywords

Stichodactyla haddoni Adhesive protein Antibiofilm Antioxidant Insecticide 

Notes

Acknowledgements

The authors appreciatively acknowledge the financial support of RUSA-Phase 2.0-F.24-51/2014-U (TN Multi-Gen), Dept. of Edn, Govt of India. MA gratitude the support of DST-INSPIRE fellowship-IF160623, New Delhi, India. The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through research group No. RG-1438-074. The authors thank the Deanship of Scientific Research and RSSU at King Saud University for their technical support.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Muthukumar Abinaya
    • 1
  • Ravichandran Rekha
    • 1
  • Shanthini Sivakumar
    • 2
  • Marimuthu Govindarajan
    • 3
    • 4
  • Naiyf S. Alharbi
    • 5
  • Shine Kadaikunnan
    • 5
  • Jamal M. Khaled
    • 5
  • Ahmed S. Alobaidi
    • 5
  • Mohammed N. Al-Anbr
    • 5
  • Baskaralingam Vaseeharan
    • 1
    Email author
  1. 1.Nano Biosciences and Nano Pharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and ManagementAlagappa UniversityKaraikudiIndia
  2. 2.Centre for Animal Science Research and Extension ServicesFoundation for Innovative Research in Science and TechnologyNagercoilIndia
  3. 3.Unit of Vector Control, Phytochemistry and Nanotechnology, Department of ZoologyAnnamalai UniversityAnnamalainagarIndia
  4. 4.Department of ZoologyGovernment College for Women (Autonomous)KumbakonamIndia
  5. 5.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia

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