Antibacterial Activity of Alkaloids, Flavonoids, Saponins and Tannins Mediated Green Synthesised Silver Nanoparticles Against Pseudomonas aeruginosa and Bacillus subtilis

  • P. Raji
  • Antony V. SamrotEmail author
  • D. Keerthana
  • S. Karishma
Original Paper


In this study, metabolites like tannin, saponin, alkaloids and flavonoids from Cassia alata, Euphorbia hirta, Thespesia populnea and Wrightia tinctoria were utilized for green synthesize of silver nanoparticles, where crude extracts were commonly used. The silver nanoparticles produced using the metabolites were crystalline and found to be spherical and rod shaped with size range between 17 and 30 nm. All the silver nanoparticles were effective against the microorganisms used in this study. Minimum inhibitory concentration of silver nanoparticles produced using flavonoid and tannin of E. hirta and saponin of C. alata, was found to be at 0.5 µg concentration against Pseudomonas aeruginosa and Bacillus subtilis. All the nanoparticles were able to disturb the cell membrane and released out internal protein and also showing impact on swarming motility of both the bacteria used in this study.


Medicinal plants Alkaloids Flavonoids Saponins Tannins Silver nanoparticles Antibacterial activity 


Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Department of BiotechnologySathyabama Institute of Science and TechnologyChennaiIndia
  2. 2.Department of Biomedical Sciences, Faculty of Medicine and Biomedical SciencesMAHSA UniversityJenjaromMalaysia

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