Journal of Cluster Science

, Volume 30, Issue 1, pp 181–196 | Cite as

Fungicidal Efficiency of Silver and Copper Nanoparticles Produced by Pseudomonas fluorescens ATCC 17397 Against Four Aspergillus Species: A Molecular Study

  • Eman Zakaria GomaaEmail author
  • Manal Maher Housseiny
  • Ahmed Ali Abdel-Khalik Omran
Original Paper


Nano-science has emerged as a powerful tool in the field of designing new antimicrobial substances. In the present study, a green and eco-friendly method for synthesizing silver and copper nanoparticles using Pseudomonas fluorescens was employed. The biosynthesis of metals nanoparticles was firstly identified by the color change of the extracellular culture filtrate and confirmed with the help of the study of UV–Vis spectroscopy. The synthesized nanoparticles have further been characterized by transmission electron microscopy, energy dispersive X-ray and fourier transformation infrared studies. The antifungal activity of AgNPs and CuNPs was evaluated in vitro and the results showed that AgNPs had the greater effect as compared to CuNPs and Aspergillus species were the most sensitive strains. Protein electrophoresis results of Aspergillus species demonstrated the change in the protein profiles of the four fungal species against their controls after treatment with AgNPs and CuNPs. In addition, ISSR PCR analysis using five primers (HB-08, 44B, HB-10, HB-15 and 98A) indicated the appearance and disappearance of DNA polymorphic bands and induction of a high genetic variability in response to the two treatments. The results confirmed that metal nanoparticles are a promising approach to control fungal pathogens and eliminate their toxicity; therefore their applications should gained significant importance.


Silver nanoparticles Copper Nanoparticles Characterization Antifungal activity Protein electrophoresis ISSR PCR 


Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Statement

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Eman Zakaria Gomaa
    • 1
    Email author
  • Manal Maher Housseiny
    • 1
  • Ahmed Ali Abdel-Khalik Omran
    • 1
  1. 1.Department of Biological and Geological Sciences, Faculty of EducationAin Shams UniversityCairoEgypt

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