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Imbalanced Expression of T-bet and T Cell Immunoglobulin Mucin-3 in Patients with Aplastic Anaemia

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Abstract

Background

Activated T helper (Th)-1 CD4+ cells and their mediators are essential for pathogenesis processes in aplastic anaemia (AA). Recently, T-cell immunoglobulin and mucin domain 3 (Tim-3) molecules, a Th1-specific type 1 membrane protein, have been suggested to be important regulators of both Th1 proliferation and the development of tolerance. Moreover, T-box expressed in T cells (T-bet) is a major T cell transcription factor that regulates the expression of Th1 cytokine genes and plays a crucial role in T cell differentiation. The function of Tim-3 and its association with T-bet in the pathophysiology of AA remain unclear.

Design and Methods

Plasma IL-18, IFN-γ and IL-4 levels were measured in patients with newly diagnosed AA (n = 29), AA in remission (n = 22) and healthy subjects (n = 30) via enzyme-linked immunosorbent assay (ELISA). CD4+ Tim-3+ cells were evaluated via flow cytometry and expressed as a percentage of the total number of CD4+ cells. Using real-time quantitative polymerase chain reaction (RT-PCR) and mRNA expression analysis the expression levels of Tim-3, IL-18, IFN-γ and T-box (T-bet) were examined in all subjects.

Results

Tim-3 was expressed on CD4+T cells. The percentages of Tim-3 cells identified in newly diagnosed patients were significantly deceased compared with the controls. Meanwhile T-bet, IL-18 and IFN-γ levels were significantly elevated in patients, which resulted in an increased ratio of T-bet/Tim-3 expression levels in patients with active disease. During the remission stages, the levels of these cytokines were comparable with those observed in the healthy controls.

Conclusions

These results suggest that the imbalanced expression of Tim-3 and T-bet may play a role in the pathogenesis and course of AA, and the downregulation of T-bet/Tim-3 may represent a reasonable therapeutic strategy for AA treatment.

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Funding

This study was partially supported by research funding from the National Science Foundation of China (No. 81100334), Natural Science Foundation of Shandong Province (ZR2009CM001, ZR2010HQ002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests

The authors have declared no competing interests exist.

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Authors and Affiliations

  1. Department of Hematology, Provincial Hospital Affiliated to Shandong University, 324 Jing Wu Rd, Jinan, Shandong, 250021, People’s Republic of China

    Ning-ning Shan, Xin Liu, Xin Wang, Dai Yuan & Ying Li

  2. Haematology Oncology Centre, Qilu Hospital, Shandong University, Jinan, 250012, China

    Yu Hu

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  1. Ning-ning Shan
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  2. Yu Hu
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  3. Xin Liu
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  4. Xin Wang
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Corresponding author

Correspondence to Xin Wang.

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Yu Hu should be considered as equal first author.

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Shan, Nn., Hu, Y., Liu, X. et al. Imbalanced Expression of T-bet and T Cell Immunoglobulin Mucin-3 in Patients with Aplastic Anaemia. J Clin Immunol 33, 809–816 (2013). https://doi.org/10.1007/s10875-013-9864-7

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  • DOI: https://doi.org/10.1007/s10875-013-9864-7

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