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Experimental measurements of the sinking speed of sperm of an acroporid coral, Acropora tenuis, in static seawater

  • Tokihiro KonoEmail author
  • Ryota Nakamura
  • Makoto Omori
Original Article

Abstract

The dispersion of Acropora coral sperm is critical for sexual reproduction via external fertilization. Sperm sinking likely plays an important role in the process of dispersion, but its rate of sinking is still unclear. To estimate the sinking speed of coral sperm without turbulence, two experiments were conducted in a water tank filled with 500 L of static seawater in the 0–60 cm layer. In Exp. 1, suspension of sperm was gently poured from a 2 L plastic beaker onto the surface of the seawater in the tank. In Exp. 2, sperm were released from egg–sperm bundles from coral colonies at the bottom of the tank. Sinking speeds ranged between 0 and 120 cm h−1. In the sperm sinking experiment (Exp. 1), 43% of sperm remained on the surface layer, while the remainder sank 30–180 min after being poured. Sperm that formed clusters might sink faster. Although the effect of turbulence on the sinking speed of sperm in nature has not yet been clarified, the mean residence time of sperm in the 0–10 cm layer (Exp 2; 0.5–0.7 h) implies that sinking speed inherited in sperm is linked to gamete interactions for fertilization.

Keywords

Acropora tenuis Sperm Sinking speed Tank experiment Vertical distribution Coral spawning 

Notes

Acknowledgements

This work was supported by ordinary research fund from Tokai University for English proofreading by Editage.

References

  1. Arai I, Kato M, Heyward A, Ikeda Y, Iizuka T, Maruyama T (1993) Lipid composition of positively buoyant eggs of reef building corals. Coral Reefs 12:71–75CrossRefGoogle Scholar
  2. Ayre DJ, Resing JM (1986) Sexual and asexual production of planulae in reef corals. Mar Biol 90:187–190CrossRefGoogle Scholar
  3. Babcock RC, Heyward AJ (1986) Larval development of certain gamete-spawning scleractinian corals. Coral Reefs 5:111–116CrossRefGoogle Scholar
  4. Babcock RC, Bull GD, Harrison PL, Heyward AJ, Oliver JK, Wallace CC, Willis BL (1986) Synchronous spawnings of 105 scleractinian coral species on the Great Barrier Reef. Mar Biol 90:379–394CrossRefGoogle Scholar
  5. Bach LT, Riebesell U, Sett S, Febiri S, Rzepka P, Schulz KZ, Febiri S, Rzepka P, Schulz KG (2012) An approach for particle sinking velocity measurements in the 3–400 μm size range and considerations on the effect of temperature on sinking rates. Mar Biol 159:1853–1864CrossRefGoogle Scholar
  6. Bach LT, Boxhammer T, Larsen A, Hildebrandt N, Schulz KG, Riebesell U (2016) Influence of plankton community structure on the sinking velocity of marine aggregates. Glob Biogeochem Cycles 30:1145–1165CrossRefGoogle Scholar
  7. Baird AH, Guest JR, Willis BL (2009) Systematic and biogeographical patterns in the reproductive biology of scleractinian corals. Ann Rev Ecol Evol Syst 40:551–571CrossRefGoogle Scholar
  8. Berke AP, Turner L, Berg HC, Lauga E (2008) Hydrodynamic attraction of swimming microorganisms by surfaces. Phys Rev Lett 101:038102.  https://doi.org/10.1103/PhysRevLett.101.038102 CrossRefGoogle Scholar
  9. Boryshpolets S, Cosson J, Bondarenko V, Gillies E, Rodina M, Dzyuba B, Linhart O (2013) Different swimming behaviors of sterlet (Acipenser ruthenus) spermatozoa close to solid and free surfaces. Theriogenology 79:81–86CrossRefGoogle Scholar
  10. Chang H, Kim BJ, Kim YS, Suarez SS, Wu M (2013) Different migration patterns of sea urchin and mouse sperm revealed by a microfluidic chemotaxis device. PLoS One 8:e60587.  https://doi.org/10.1371/journal.pone.0060587 CrossRefGoogle Scholar
  11. Chui APY, Wong MC, Liu SH, Lee GW, Chan SW, Lau PL, Leung SM, Put A Jr (2014) Gametogeneisis, embryogenesis, and fertilization ecology of Platygyra acuta in marginal nonreefal coral communities in Hong Kong. J Mar Biol.  https://doi.org/10.1155/2014/953587 CrossRefGoogle Scholar
  12. Dunson DB, Weinberg CR, Perreault SD, Chapin RE (1999) Summarizing the motion of self-propelled cells: applications to sperm motility. Biometrics 55:537–543CrossRefGoogle Scholar
  13. Durkin CA, Estapa ML, Buesseler KO (2015) Observations of carbon export by small sinking particles in the upper mesopelagic. Mar Chem 175:72–81CrossRefGoogle Scholar
  14. Falkenberg LJ, Havenhand JN, Styan CA (2016) Sperm accumulated against surface: a novel alternative bioassay for environmental monitoring. Mar Environ Res 114:51–57CrossRefGoogle Scholar
  15. Fisher HB, List EJ, Koh RCY, Imberger J, Brooks NH (1979) Mixing in inland and coastal waters. Academic Press, San Diego, p 302Google Scholar
  16. Geyer WR, Morris JT, Prahl FG, Jay DA (2000) Interaction between physical processes and ecosystem structure: a comparative approach. In: Hobbie JE (ed) Estuarine science: a synthetic approach to research and practice. Island Press, Washington DC, pp 177–206Google Scholar
  17. Harrison PL, Wallace C (1990) Reproduction, dispersal and recruitment of scleractinian corals. In: Dubinsky Z (ed) Coral reefs. Elsevier, Amsterdam, pp 133–207Google Scholar
  18. Hayashibara T, Shimoike K, Kimura T, Hosaka S, Heyward A, Harrison P, Kudo K, Omori M (1993) Patterns of coral spawning at Akajima Island, Okinawa, Japan. Mar Ecol Prog Ser 101:253–262CrossRefGoogle Scholar
  19. Hill PS, Nowell ARM, Jumars PA (1992) Encounter rate by turbulent shear of particles similar in diameter to the Kolmogorov scale. J Mar Res 50:643–668CrossRefGoogle Scholar
  20. Iguchi A, Morita M, Nakajima Y, Nishikawa A, Miller D (2009) In vitro fertilization efficiency in coral Acropora digitifera. Zygote 17:225–227CrossRefGoogle Scholar
  21. Iwao K, Omori M, Taniguchi H, Tamura M (2010) Transplanted Acropora tenuis (Dana) spawned first in their life 4 years after culture from eggs. Glaxea J Coral Reef Stu 11:38Google Scholar
  22. Kapral R (2008) Multiparticle collision dynamics: simulation of complex systems on mesoscales. Adv Chem Phys 140:89–146Google Scholar
  23. Kinzie RA III, Buddemeier RW (1996) Reefs happen. Glob Chang Biol 2:479–494CrossRefGoogle Scholar
  24. Knauss JA (2017) Introduction to physical oceanography. Waveland Press Inc, Long Grove, p 310Google Scholar
  25. Levitan DR, Petersen C (1995) Sperm limitation in the sea. Trends Ecol Evol 10:228–231CrossRefGoogle Scholar
  26. Matsukawa Y, Suzuki T (1985) Box model analysis of hydrography and behaviour of nitrogen and phosphorus in a eutrophic estuary. J Oceanogr Soc Japan 41:407–426CrossRefGoogle Scholar
  27. Meyer T, Schindler H (1988) Particle counting by fluorescence correlation spectroscopy. Biophys J 54:983–993CrossRefGoogle Scholar
  28. Monsen NE, Cloern JE, Lucas LV, Monismith SG (2002) A comment on the use of flushing time, residence time, and age as transport time scales. Limnol Oceanogr 47:1545–1553CrossRefGoogle Scholar
  29. Morita M, Nishikawa A, Nakajima A, Iguchi A, Sakai A, Takemura A, Okuno M (2006) Eggs regulate sperm flagella motility initiation, chemotaxis and inhibition in the coral Acropora digitifera, A. gemmifera and A. tenuis. J Exp Biol 209:4574–4579CrossRefGoogle Scholar
  30. Nakamura R, Ando W, Yamamoto H, Kitano M, Sato A, Nakamura M, Kayanne H, Omori M (2011) Corals mass-cultured from eggs and transplanted as juveniles to their native, remote coral reef. Mar Ecol Prog Ser 436:161–168CrossRefGoogle Scholar
  31. Nozawa Y, Isomura N, Fukami H (2015) Influence of sperm dilution and gamete contact time on the fertilization rate of scleractinian corals. Coral Reefs 34:1199–1206CrossRefGoogle Scholar
  32. Oliver J, Babcock R (1992) Aspects of the fertilization ecology of broadcast spawning corals: sperm dilution effects and in situ measurements of fertilization. Biol Bull 183:409–417CrossRefGoogle Scholar
  33. Omori M, Fukami H, Kobinata H, Hatta M (2001) Significant drop of fertilization of Acropora corals in 1999: an after-effect of heavy coral bleaching? Limnol Oceanogr 46:704–706CrossRefGoogle Scholar
  34. Omori M, Iwao K, Tamura M (2008) Growth of transplanted Acropora tenuis 2 years after egg culture. Coral Reefs 27:165CrossRefGoogle Scholar
  35. Padilla-Gamiño JL, Weatherby TM, Waller RG, Gates RD (2011) Formation and structural organization of the egg-sperm bundle of the scleractinian coral Montipora capitate. Coral Reefs 30:371–380CrossRefGoogle Scholar
  36. Pratchett MS, Baird AH, Marquis CP (2000) Comparative palatability among eggs of mass-spawning corals. In: Proceedings 9th international coral reef symposium, Bali, pp 23–27Google Scholar
  37. Purcell EM (1977) Life at low Reynolds number. Am J Phys 45:3–11CrossRefGoogle Scholar
  38. Ricardo GF, Jones RJ, Clode PL, Humanes A, Negri AP (2015) Suspended sediments limit coral sperm availability. Sci Rep 5:18084.  https://doi.org/10.1038/srep18084 CrossRefGoogle Scholar
  39. Richmond RH (1997) Reproduction and recruitment in corals: critical links in the persistence of reefs. In: Birkeland C (ed) Life and death of coral reefs. Chapman and Hall, New York, pp 175–197CrossRefGoogle Scholar
  40. Ripple DC, DeRose PC (2018) Primary determination of particle number concentration with light obscuration and dynamic imaging particle counters. J Res Natl Inst Stan 123:123002.  https://doi.org/10.6028/jres.123.002 CrossRefGoogle Scholar
  41. Rothschild L (1963) Non-random distribution of bull spermatozoa in a drop of sperm suspension. Nature 198:1221–1222CrossRefGoogle Scholar
  42. Suzuki G, Okada W, Yasutake Y et al (2018) Interspecific differences in the post-settlement survival of Acropora corals under a common garden experiment. Fish Sci 84:849–856CrossRefGoogle Scholar
  43. Takeoka H (1984) Fundamental concepts of exchange and transport time scales in a coastal sea. Continent Shelf Res 3:311–326CrossRefGoogle Scholar
  44. Wallace CC (1985) Reproduction, recruitment and fragmentation in nine sympatric species of the coral genus Acropora. Mar Biol 88:217–233CrossRefGoogle Scholar
  45. Willis HL, Babcock RC, Harrison PL, Wallace CC (1997) Experimental hybridization and breeding incompatibilities within the mating systems of mass spawning reef corals. Coral Reefs 16:S53–S65CrossRefGoogle Scholar
  46. Wolstenholme JK (2004) Temporal reproductive isolation and gametic compatibility are evolutionary mechanisms in the Acropora humilis species group (Cnidaria; Scleractinia). Mar Biol 144:567–582CrossRefGoogle Scholar
  47. Xia H, Francois N, Punzmann H, Shats M (2014) Taylor particle dispersion during transition to fully developed. Phys Rev Lett 112:104501.  https://doi.org/10.1103/PhysRevLett.112.104501 CrossRefGoogle Scholar
  48. Yund PO (2000) How severe is sperm limitation in natural populations of marine free-spawners? Trends Ecol Evol 15:10–13CrossRefGoogle Scholar
  49. Zayasu Y, Nakajima Y, Sakai S, Suzuki G, Satoh N, Shinzato C (2016) Unexpectedly complex gradation of coral population structure in the Nansei Islands, Japan. Ecol Evol 6:5491–5505CrossRefGoogle Scholar

Copyright information

© The Oceanographic Society of Japan and Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of Marine Biology and Sciences, School of Biological SciencesTokai UniversitySapporoJapan
  2. 2.Fisheries Infrastructure Development CenterTokyoJapan
  3. 3.Akajima Marine Science LaboratoryZamamisonJapan
  4. 4.FujisawaJapan

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