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Heat damage of cytoskeleton in erythrocytes increases membrane roughness and cell rigidity

  • E. Yu ParshinaEmail author
  • A. I. Yusipovich
  • A. R. Brazhe
  • M. A. Silicheva
  • G. V. Maksimov
Original Paper

Abstract

The intensity of erythrocyte membrane fluctuations was studied by laser interference microscopy (LIM), which provide information about mechanical properties of the erythrocyte membrane. Atomic force microscopy (AFM) was used to study erythrocyte surface relief; it is related to the cytoskeleton structure of erythrocyte membrane. Intact human erythrocytes and erythrocytes with a destroyed cytoskeleton were used. According to the obtained results, cytoskeleton damage induced by heating up to 50 °С results in a reduced intensity of cell membrane fluctuations compared to non-treated cells (20.6 ± 10.2 vs. 30.5 ± 5.5 nm, correspondingly), while the roughness of the membrane increases (4.5 ± 1.5 vs. 3.4 ± 0.5 nm, correspondingly).

Keywords

Erythrocytes  Cytoskeleton  Roughness  Membrane fluctuations  Elastic properties 

Notes

Acknowledgments

This work was supported by Russian Foundation for Basic Research (grant #17-00-00407)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Biological DepartmentM.V. Lomonosov Moscow State UniversityMoscowRussia

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