Is a constant low-entropy process at the root of glycolytic oscillations?
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We measured temporal oscillations in thermodynamic variables such as temperature, heat flux, and cellular volume in suspensions of non-dividing yeast cells which exhibit temporal glycolytic oscillations. Oscillations in these variables have the same frequency as oscillations in the activity of intracellular metabolites, suggesting strong coupling between them. These results can be interpreted in light of a recently proposed theoretical formalism in which isentropic thermodynamic systems can display coupled oscillations in all extensive and intensive variables, reminiscent of adiabatic waves. This interpretation suggests that oscillations may be a consequence of the requirement of living cells for a constant low-entropy state while simultaneously performing biochemical transformations, i.e., remaining metabolically active. This hypothesis, which is in line with the view of the cellular interior as a highly structured and near equilibrium system where energy inputs can be low and sustain regular oscillatory regimes, calls into question the notion that metabolic processes are essentially dissipative.
KeywordsGlycolytic oscillations Isentropic process Temperature oscillations Onsager’s theory Association-induction hypothesis
HST and LFO thank the Danish Council for Independent Research|Natural Sciences for support. LAB is a member of the Argentinian Research Council (CONICET) research career. The authors thank Anita Lunding for skilled technical assistance.
This study was funded by a grant from the Danish Council for Independent Research|Natural Sciences (grant # DFF - 4002-00465).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- 7.Lokta, A.J.: Contribution to the theory of periodic reactions. J. Phys. Chem. 14(3), 271–274 (1910)Google Scholar
- 10.Ytting, C.K., Fuglsang, A.T., Hiltunen, J.K., Kastaniotis, A.J., Ozalp, V.C., Nielsen, L.J., Olsen, L.F.: Measurements of intracellular ATP provide new insight into the regulation of glycolysis in the yeast Saccharomyces cerevisiae. Integr. Biol. (Camb) 4(1), 99–107 (2012). https://doi.org/10.1039/c1ib00108f
- 24.Ling, G.N.: A Physical Theory of the Living State: the Association-Induction Hypothesis. Blaisdell Publishing Co, A Division of Random House, Inc., New York (1962)Google Scholar
- 25.Jaeken, L., Matveev, V.V.: Coherent behaviour and the bound state of water and K+ imply another model of bioenergetics: negative entropy instead of high energy bonds. The Open Biochemistry Journal 6, 139–159 (2012)Google Scholar
- 26.Kondepudi, D., Prigogine, I.: Modern Thermodynamics. From Heat Engines to Dissipative Structures. John Wiley & Sons Ltd,. Chichester (1998)Google Scholar
- 32.Richard, P., Teusink, B., Hemker, M.B., Van Dam, K., Westerhoff, H.V.: Sustained oscillations in free-energy state and hexose phosphates in yeast. Yeast 12(8), 731–740 (1996). https://doi.org/10.1002/(SICI)1097-0061(19960630)12:8<731::AID-YEA961>3.0.CO;2-Z
- 33.Bagatolli, L.A., Stock, R.P.: The cell as a gel: material for a conceptual discussion. Physiological Mini Reviews 9(5), 38–49 (2016)Google Scholar
- 35.Bockmann, M., Hess, B., Muller, S.C.: Temperature gradients traveling with chemical waves. Phys. Rev. E 53(5), 5498–5501 (1996)Google Scholar
- 37.Franck, U.F.: Chemical Oscillations. Angewandte Chemie-International 17, 1–15 (1978)Google Scholar
- 38.Wang, T.: Studies on the action potential from a thermodynamic perspective. University of Copenhagen (2017)Google Scholar
- 40.Schrödinger, E.: What is Life – the Physical Aspect of the Living Cell. Cambridge University Press (1944)Google Scholar
- 41.Ling, G.N.: Life at the cell and below cell level. The hidden history of a fundamental revolution in biology. Pacific Press, (2001)Google Scholar