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Journal of Biological Physics

, Volume 44, Issue 3, pp 301–315 | Cite as

Toxicity impact of fenvalerate on the gill tissue of Oreochromis mossambicus with respect to biochemical changes utilizing FTIR and principal component analysis

  • B. Velmurugan
  • P. Senthilkumaar
  • S. Karthikeyan
Original Paper

Abstract

The use of pesticides in agriculture can make their way into the earth and wash into the amphibian system causing ecological stress. This study aims to understand the changes occurring in gill tissues as a result of fenvalerate exposure using Fourier-transform infrared spectroscopy. The intensity ratio of the selected bands I1545/I1657, I2924/I2853, and I1045/I1545 measures changes in proteins, lipids, and carbohydrates. Curve-fitting analysis was performed in the selected band region to analyze the quantitative changes of proteins, lipids, and carbohydrates. The band area ratio of CH3/asCH2+ sCH2 shows the absence of a long chain of fatty acids due to fenvalerate treatment. The band area ratio of asCH2/sCH2 increases for higher sublethal concentrations, which shows the lower disorder of lipid acyl chain flexibility. A decrease in lipids was found in lower sublethal concentrations. The secondary structure of proteins affirms β sheet development. Carbohydrate metabolism of gill tissues demonstrates a decrease in glycogen contents. A further decrease in glycogen content and an increase in lactic acid were observed when presented to a fenvalerate concentration. PCA plots indicate distinct variations among the biochemical parameters of the gill tissues. This study provides a quantitative examination of assessing pesticide toxicity in aquatic environments.

Keywords

Toxicity Pesticides Fish FTIR PCA Proteins Lipids Carbohydrates 

Notes

Acknowledgements

The authors thank S.A.I.F, I.I.T, Chennai, for help with the recording of the FTIR spectra.

Compliance with Ethical Standards

Conflict of interest

There are no known conflicts of interest among the authors associated with this work.

Ethical approval

We do not require ethical approval for carrying out experiments with fishes in India.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.P.G. & Research Department of ZoologySir Theagaraya CollegeChennaiIndia
  2. 2.Department of PhysicsDr. Ambedkar Government Arts CollegeChennaiIndia

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