MAS dependent sensitivity of different isotopomers in selectively methyl protonated protein samples in solid state NMR
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Sensitivity and resolution together determine the quality of NMR spectra in biological solids. For high-resolution structure determination with solid-state NMR, proton-detection emerged as an attractive strategy in the last few years. Recent progress in probe technology has extended the range of available MAS frequencies up to above 100 kHz, enabling the detection of resolved resonances from sidechain protons, which are important reporters of structure. Here we characterise the interplay between MAS frequency in the newly available range of 70–110 kHz and proton content on the spectral quality obtainable on a 1 GHz spectrometer for methyl resonances. Variable degrees of proton densities are tested on microcrystalline samples of the α-spectrin SH3 domain with selectively protonated methyl isotopomers (CH3, CH2D, CHD2) in a perdeuterated matrix. The experimental results are supported by simulations that allow the prediction of the sensitivity outside this experimental frequency window. Our results facilitate the selection of the appropriate labelling scheme at a given MAS rotation frequency.
KeywordsSolid state NMR Magic angle spinning (MAS) Selective deuteration CH3 labelling Methyl isotopomers Microcrystalline proteins
This work was performed in the framework of the SFB-1035 (Project B07; German Research Foundation, DFG). We acknowledge support from the Helmholtz-Gemeinschaft, the Deutsche Forschungsgemeinschaft (DFG, Grant Re1435), the Center for Integrated Protein Science Munich (CIPS-M), the CNRS (IR-RMN FR3050), the European Research Council (ERC) (ERC-2014-CoG “P-MEM-NMR” GA n 648974) and by the EU access project iNext (GA 653706).
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