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Journal of Biomolecular NMR

, Volume 73, Issue 10–11, pp 519–523 | Cite as

Feasibility of trifluoromethyl TROSY NMR at high magnetic fields

  • Brittney A. Klein
  • Brian D. SykesEmail author
Communication
  • 295 Downloads

Abstract

Insights into the structure and dynamics of large biological systems has been greatly improved by two concurrent NMR approaches: the application of transverse relaxation-optimized spectroscopy (TROSY) techniques in multi-dimensional NMR, especially the methyl-TROSY, and the resurgence of 19F NMR using trifluoromethyl (CF3) probes. Herein we investigate the feasibility of combining these approaches into a trifluoromethyl-TROSY experiment. Using a CF3-labelled parvalbumin, we have evaluated the natural abundance 13C-19F correlation spectra and find no indication of a CF3 TROSY at high magnetic fields.

Keywords

Methyl-TROSY Trifluoromethyl 19F NMR Chemical shift anisotropy High magnetic fields 

Notes

Acknowledgements

We would like to thank Dr. Jack Moore of the Alberta Proteomics and Mass Spectrometry Facility for assistance with mass spectrometry of proteins and Dr. Andrew Atkinson at King’s College London for 400 MHz NMR spectra.

Author contributions

BAK, BDS contributed equally to the execution of these experiments and the writing of the paper.

Funding

This research has been supported by grants from The Heart and Stroke Foundation of Canada (G-14-0005884, BDS) and Faculty of Medicine Transitional Program.

Supplementary material

10858_2019_266_MOESM1_ESM.pdf (6 mb)
Supporting Information.Protocol for 19F-labelling carp-β parvalbumin, NMR experimental conditions and Supplementary Figures S1-7. (PDF 6131 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of AlbertaEdmontonCanada

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