Osteogenic potential of sol–gel bioactive glasses containing manganese
- 58 Downloads
Bioactive glasses (BGs) are widely used for bone regeneration, and allow the incorporation of different ions with therapeutic properties into the glass network. Amongst the different ions with therapeutic benefits, manganese (Mn) has been shown to influence bone metabolism and activate human osteoblasts integrins, improving cell adhesion, proliferation and spreading. Mn has also been incorporated into bioceramics as a therapeutic ion for improved osteogenesis. Here, up to 4.4 mol% MnO was substituted for CaO in the 58S composition (60 mol% SiO2, 36 mol% CaO, 4 mol% P2O5) and its effects on the glass properties and capability to influence the osteogenic differentiation were evaluated. Mn-containing BGs with amorphous structure, high specific surface area and nanoporosity were obtained. The presence of Mn2+ species was confirmed by X-ray photoelectron spectroscopy (XPS). Mn-containing BGs presented no cytotoxic effect on human mesenchymal stem cells (hMSCs) and enabled sustained ion release in culture medium. hMSCs osteogenic differentiation stimulation and influence on the mineralisation process was also confirmed through the alkaline phosphatase (ALP) activity, and expression of osteogenic differentiation markers, such as collagen type I, osteopontin and osteocalcin, which presented higher expression in the presence of Mn-containing samples compared to control. Results show that the release of manganese ions from bioactive glass provoked human mesenchymal stem cell (hMSC) differentiation down a bone pathway, whereas hMSCs exposed to the Mn-free glass did not differentiate. Mn incorporation offers great promise for obtaining glasses with superior properties for bone tissue regeneration.
The authors acknowledge financial support from CNPq, CAPES and FAPEMIG/Brazil and to the Advanced Photoelectron Spectroscopy Laboratory (APSL—Department of Materials—Imperial College London) for XPS analysis.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 4.Xynos ID, Edgar AJ, Buttery LDK, Hench LL, Polak JM. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution. J Biomed Mater Res. 2001;55:151–7. https://doi.org/10.1002/1097-4636(200105)55:2<151::AID-JBM1001>3.0.CO;2-D.CrossRefGoogle Scholar
- 5.Bosetti M, Cannas M. The effect of bioactive glasses on bone marrow stromal cells differentiation. Biomaterials. 2005;26:3873–9. https://doi.org/10.1016/j.biomaterials.2004.09.059.CrossRefGoogle Scholar
- 6.Reilly GC, Radin S, Chen AT, Ducheyne P. Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bioactive glass. Biomaterials. 2007;28:4091–7. https://doi.org/10.1016/j.biomaterials.2007.05.038.CrossRefGoogle Scholar
- 13.Gentleman E, Fredholm YC, Jell G, Lotfibakhshaiesh N, O’Donnell MD, Hill RG et al. The effects of strontium-substituted bioactive glasses on osteoblasts and osteoclasts in vitro. Biomaterials. 2010;31:3949–56. https://doi.org/10.1016/j.biomaterials.2010.01.121.CrossRefGoogle Scholar
- 24.Kargozar S, Lotfibakhshaiesh N, Ai J, Mozafari M, Brouki Milan P, Hamzehlou S et al. Strontium- and cobalt-substituted bioactive glasses seeded with human umbilical cord perivascular cells to promote bone regeneration via enhanced osteogenic and angiogenic activities. Acta Biomater. 2017;58:502–14. https://doi.org/10.1016/j.actbio.2017.06.021.CrossRefGoogle Scholar
- 32.Nawaz Q, Atiq M, Rehman U, Burkovski A, Schmidt J, Beltrán AM. et al., Synthesis and characterization of manganese containing mesoporous bioactive glass nanoparticles for biomedical applications. J. Mater. Sci. Mater. Med. 2018;5: https://doi.org/10.1007/s10856-018-6070-4.
- 38.de Oliveira AAR, de Souza DA, Dias LLS, de Carvalho SM, Mansur HS, de Magalhães M. Pereira, Synthesis, characterization and cytocompatibility of spherical bioactive glass nanoparticles for potential hard tissue engineering applications. Biomed Mater. 2013;8:025011 https://doi.org/10.1088/1748-6041/8/2/025011.CrossRefGoogle Scholar
- 41.Atkinson I, Anghel EM, Predoana L, Mocioiu OC, Jecu L, Raut I et al. Influence of ZnO addition on the structural, in vitro behavior and antimicrobial activity of sol–gel derived CaO–P2O5–SiO2 bioactive glasses. Ceram Int. 2016;42:3033–45. https://doi.org/10.1016/j.ceramint.2015.10.090.CrossRefGoogle Scholar
- 42.Gunawidjaja PN, Mathew R, Lo aYH, Izquierdo-Barba I, Garcia a, Arcos D et al. Local structures of mesoporous bioactive glasses and their surface alterations in vitro: inferences from solid-state nuclear magnetic resonance. Philos Trans R Soc A Math Phys Eng Sci. 2012;370:1376–99. https://doi.org/10.1098/rsta.2011.0257.CrossRefGoogle Scholar
- 59.Regan E, Groutso T, Metson JB, Steiner R, Ammundsen B, Hassell D et al. Surface and bulk composition of lithium manganese oxides. Surf Interface Anal. 1999;27:1064–8. https://doi.org/10.1002/(SICI)1096-9918(199912)27:12<1064::AID-SIA676>3.0.CO;2-S.CrossRefGoogle Scholar
- 69.LA Strobel, N Hild, D Mohn, WJ Stark, A Hoppe, U Gbureck, RE Horch, U Kneser, AR Boccaccini, Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells. J Nanopart Res. 2013;15. https://doi.org/10.1007/s11051-013-1780-5.