FGF adsorbed mesoporous bioactive glass with larger pores in enhancing bone tissue engineering
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Mesoporous bioactive glass (MBG) is performed as a bone tissue engineering material because of its good bioactivity, biocompatibility and osteoinducion characteristics. Here, we propose MBG with larger pores (MBG-L) adsorbed fibroblast growth factor (FGF) to facilitate osteoblast differentiation and matrix mineralization. Specifically, we observed that MBG-L promotes calcium deposit precipitation in vitro. In addition, adhesion, proliferation, differentiation and matrix mineralization were promoted after osteoblast cultured on MBG-L/FGF. Interestingly, we found that the transcriptional activity of the critical transcription factor Runx2 was increased through MAPK pathway after osteoblast cultured on MBG-L/FGF. Support for this result, we found that the expression of osteoblastic marker genes, Osteocalcin (Ocn), Osteopontin (Opn), and Runx2 were increased. Thus, our findings provided that MBG-L/FGF could be a promising new material in bone tissue engineering.
We thank Dr. RT Franceschi for kindly providing 1.3 kb mOG2-Luc plasmids. This work was supported by the Research Program of National University of Defense Technology (ZK16-03-15) and Nature Science Foundation of Hunan Province (2018JJ3598).
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Conflict of interest
The authors declare that they have no conflict of interest.
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