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Impact of sterilization by electron beam, gamma radiation and X-rays on electrospun poly-(ε-caprolactone) fiber mats

  • Dominik de Cassan
  • Anna Lena Hoheisel
  • Birgit Glasmacher
  • Henning MenzelEmail author
Biomaterials Synthesis and Characterization Original Research
  • 45 Downloads
Part of the following topical collections:
  1. Biomaterials Synthesis and Characterization

Abstract

Biodegradable polymers such as polycaprolactone (PCL) are increasingly used for electrospinning substrates for tissue engineering. These materials offer great advantages such as biocompatibility and good mechanical properties. However, in order to be approved for human implantation they have to be sterilized. The impact of commonly used irradiation sterilization methods on electrospun PCL fiber mats was investigated systematically. Electron beam (β-irradiation), gamma and X-ray irradiation with two different doses (25 and 33 kGy) were investigated. To determine the impact on the fiber mats, mechanical, chemical, thermal properties and crystallinity were investigated. Irradiation resulted in a significant decrease in molecular weight. At the same time, crystallinity of fiber mats increased significantly. However, the mechanical properties did not change significantly upon irradiation, mostly likely because effects of a lower molecular weight were balanced with the higher degree of crystallinity. The irradiation effects were dose dependent, a higher irradiation dose led to stronger changes. Gamma irradiation seemed to be the least suited method, while electron beams (β irradiation) had a lower impact. Therefore, β irradiation is recommended as sterilization method for electrospun PCL fiber mats.

Notes

Acknowledgements

This research project has been supported by the DFG FOR 2180 “Gradierte Implantate für Sehnen-Knochen-Verbindungen“. Furthermore the authors would like to thank “Thünen-Institut für Agrartechnologie” for assistance in performing DSC measurements. The authors would like to thank Michael Bode and Alexander Becker (Institute for Multiphase Processes, Gottfried Wilhelm Leibniz Universität Hannover, Hannover, Germany) for discussions.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute for Technical ChemistryBraunschweig University of TechnologyBraunschweigGermany
  2. 2.Institute for Multiphase ProcessesGottfried Wilhelm Leibniz Universität HannoverHannoverGermany
  3. 3.Lower Saxony Centre for Biomedical EngineeringImplant Research and Development (NIFE)HannoverGermany

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