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Osseointegration of titanium scaffolds manufactured by selective laser melting in rabbit femur defect model

  • Aranka Ilea
  • Oana-Gabriela Vrabie
  • Anida-Maria Băbțan
  • Viorel Miclăuş
  • Flavia Ruxanda
  • Melinda Sárközi
  • Lucian Barbu-Tudoran
  • Voicu Mager
  • Cristian Berce
  • Bianca Adina BoșcaEmail author
  • Nausica Bianca Petrescu
  • Oana Cadar
  • Radu Septimiu Câmpian
  • Réka Barabás
Tissue Engineering Constructs and Cell Substrates Original Research
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

The aim of this study was to assess the osseointegration of two series of titanium (Ti) scaffolds with 0.8 and 1 mm cell size obtained by Selective Laser Melting (SLM) technique. One of the series had the Ti surface unmodified, while the other had the Ti surface coated with silicon-substituted nano-hydroxyapatite (nano-HapSi). The scaffolds were implanted in the femur bone defects of 6 White Californian male rabbits: three animals were implanted with 0.8 mm cell size scaffolds and three animals with 1 mm cell size scaffolds, respectively. The bone fragments and scaffolds harvested at 2, 4 and 6 months were histologically analyzed using conventional light microscopy (LM) and scanning electron microscopy (SEM) for the qualitative evaluation of the bone tissue formed in contact with the scaffold. Both LM and SEM images indicated a better osseointegration for nano-HapSi coated Ti scaffolds. LM revealed that the compact bone formed in the proximity of nano-HapSi-coated scaffolds was better organized than spongy bone associated with unmodified scaffolds. Moreover, Ti scaffolds with meshes of 0.8 mm showed higher osseointegration compared with 1 mm. SEM images at 6 months revealed that the bone developed not only in contact with the scaffolds, but also proliferated inside the meshes. Nano-HapSi-coated Ti implants with 0.8 mm meshes were completely covered and filled with new bone. Ti scaffolds osseointegration depended on the mesh size and the surface properties. Due to the biocompatibility and favorable osseointegration in bone defects, nano-HapSi-coated Ti scaffolds could be useful for anatomical reconstructions.

Notes

Acknowledgements

This study was funded by the internal grant No 4995/20/08.03.2016 within the “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania and partially by PhD Grant of “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, No 3999/01.10.2016.

Compliance with ethical standards

Conflicts of interest

The authors declare no conflicts of interest with respect to the authorship and/or publication of this article.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Aranka Ilea
    • 1
  • Oana-Gabriela Vrabie
    • 2
  • Anida-Maria Băbțan
    • 1
  • Viorel Miclăuş
    • 3
  • Flavia Ruxanda
    • 3
  • Melinda Sárközi
    • 4
  • Lucian Barbu-Tudoran
    • 5
    • 6
  • Voicu Mager
    • 7
  • Cristian Berce
    • 8
  • Bianca Adina Boșca
    • 9
    Email author
  • Nausica Bianca Petrescu
    • 1
  • Oana Cadar
    • 10
  • Radu Septimiu Câmpian
    • 1
  • Réka Barabás
    • 4
  1. 1.Department of Oral Rehabilitation, Oral Health and Dental Office Management, Faculty of Dentistry“Iuliu Hațieganu” University of Medicine and PharmacyCluj-NapocaRomania
  2. 2.Faculty of Dentistry“Iuliu Hațieganu” University of Medicine and PharmacyCluj-NapocaRomania
  3. 3.Department of Histology and EmbriologyFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-NapocaCluj-NapocaRomania
  4. 4.Department of Chemistry and Chemical Engineering, Faculty of Chemistry and Chemical EngineeringHungarian Line of Study, “Babeş Bolyai” University Cluj-NapocaCluj-NapocaRomania
  5. 5.Department of Molecular Biology and Biotechnology, Faculty of Biology“Babeş Bolyai” University Cluj-NapocaCluj-NapocaRomania
  6. 6.National Institute for Research and Development of Isotopic and Molecular TechnologiesCluj-NapocaRomania
  7. 7.Postdoctorand of Technical University of Cluj-NapocaCluj-NapocaRomania
  8. 8.Biobase Department of “Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaCluj-NapocaRomania
  9. 9.Department of Histology, Faculty of Medicine“Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-NapocaCluj-NapocaRomania
  10. 10.INCDO-INOE 2000, Research Institute for Analytical InstrumentationCluj-NapocaRomania

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