Enhanced mesenchymal stem cell proliferation through complexation of selenium/titanium nanocomposites

  • Hanaa H. AhmedEmail author
  • Hadeer A. Aglan
  • Mostafa Mabrouk
  • Ahmed A. Abd-Rabou
  • Hanan H. Beherei
Tissue Engineering Constructs and Cell Substrates Original Research
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates


The main target of this work was to explore the proliferative impact of selenium dioxide nanoparticles (SeO2) and selenium dioxide/titanium dioxide nanocomposites (Se/Ti (I), (II) and (III)) on mesenchymal stem cells (MSCs). For this purpose, SeO2 and Se/Ti (I), (II) and (III) were prepared by facile one step method and characterized by transmission electron microscopy (TEM), Zetasizer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) along with energy-dispersive X-ray spectrometry (EDX) with reference to SeO2 nanoparticles. Also, MSCs were isolated from rat bone marrow (BM-MSCs) and adipose tissue (ADSCs), propagated and characterized by flow cytometry. Thereafter, the proliferative effect of the fabricated nanomaterials was investigated by MTT assay. The TEM and DLS results, revealed that the average particle size of the suggested nanomaterials was in nanoscale. XRD pattern showed well crystalline structure for SeO2 nanoparticles and Se/Ti (I), (II) and (III) nanocomposites; the decreasing of the crystalline phase was observed by increasing the wt% of TiO2. The designed nanomaterials showed proliferative effects on MSCs with the most prominent effect exerted by 2 µg/ml of Se/Ti (III) and 5 µg/ml of Se/Ti (II) for ADSCs and 20 µg/ml of Se/Ti (II) and 10 µg/ml of Se/Ti (III) for BM-MSCs. Therefore, these newly designed nanomaterials have a promising influence on MSCs proliferation and they are recommended to be utilized in the filed of tissue engineering.



This work was financially supported by the National Research Centre, Egypt (Grant no: 11010134).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Hormones DepartmentNational Research CentreGizaEgypt
  2. 2.Stem Cells Lab, Center of Excellence for Advanced SciencesNational Research CentreGizaEgypt
  3. 3.Refractories, Ceramics and Building Materials DepartmentNational Research CentreGizaEgypt

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