Enhanced mesenchymal stem cell proliferation through complexation of selenium/titanium nanocomposites
The main target of this work was to explore the proliferative impact of selenium dioxide nanoparticles (SeO2) and selenium dioxide/titanium dioxide nanocomposites (Se/Ti (I), (II) and (III)) on mesenchymal stem cells (MSCs). For this purpose, SeO2 and Se/Ti (I), (II) and (III) were prepared by facile one step method and characterized by transmission electron microscopy (TEM), Zetasizer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) along with energy-dispersive X-ray spectrometry (EDX) with reference to SeO2 nanoparticles. Also, MSCs were isolated from rat bone marrow (BM-MSCs) and adipose tissue (ADSCs), propagated and characterized by flow cytometry. Thereafter, the proliferative effect of the fabricated nanomaterials was investigated by MTT assay. The TEM and DLS results, revealed that the average particle size of the suggested nanomaterials was in nanoscale. XRD pattern showed well crystalline structure for SeO2 nanoparticles and Se/Ti (I), (II) and (III) nanocomposites; the decreasing of the crystalline phase was observed by increasing the wt% of TiO2. The designed nanomaterials showed proliferative effects on MSCs with the most prominent effect exerted by 2 µg/ml of Se/Ti (III) and 5 µg/ml of Se/Ti (II) for ADSCs and 20 µg/ml of Se/Ti (II) and 10 µg/ml of Se/Ti (III) for BM-MSCs. Therefore, these newly designed nanomaterials have a promising influence on MSCs proliferation and they are recommended to be utilized in the filed of tissue engineering.
This work was financially supported by the National Research Centre, Egypt (Grant no: 11010134).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Yoshiura Y, Izumi H, Oyabu T, Hashiba M, Kambara T, Mizuguchi Y, Lee BW, Okada T, Tomonaga T, Myojo T, Yamamoto K, Kitajima S, Horie M, Kuroda E, Morimoto Y. Pulmonary toxicity of well-dispersed titanium dioxide nanoparticles following intratracheal instillation. J Nanopart Res. 2015;17(6):241–51.CrossRefGoogle Scholar
- 10.Hassanin KM, Abd El-Kawi SH, Hashem KS. The prospective protective effect of selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid. Int J Nanomed. 2013;8:1713–20.Google Scholar
- 18.Medvedev SP, Shevchenko AI, Zakian SM. Induced pluripotent stem cells: problems and advantages when applying them in regenerative medicine. Acta Nat. 2010;2:18–27.Google Scholar
- 33.Wang C, Xu Y, Song WG, Chang WS. Isolation and culturation, phenotype detection of rat bone marrow mesenchymal stem cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(5):466–8.Google Scholar
- 35.Lemos EMF, Patrícia SOP, Marivalda MB. 3D Nanocomposite chitosan/bioactive glass scaffolds obtained using two different routes: an evaluation of the porous structure and mechanical properties. J Quim Nova. 2016;39(4):462–6.Google Scholar
- 39.Anehosur GV, Kulkarni RD, Naik MG, Nadiger RK. Synthesis and determination of antimicrobial activity of visible light activated TiO2 nanoparticles with polymethyl methacrylate denture base resin against staphylococcus aureus. J Gerontol Geriatr Res. 2012;1:103.Google Scholar
- 45.Zhu W, Teel G, O’Brien CM, Zhuang T, Keidar M, Zhan LG. Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium. Int J Nanomed. 2015;10:7385–96.Google Scholar
- 55.Oughlis S, Changotade S, Poirier F, Cieutat AM, Rohman G, Peltzer J, Migonney V, Lataillade JJ, Lutomski D. Improved proliferation and osteogenic differentiation of human mesenchymal stem cells on a titanium biomaterial grafted with poly(sodium styrene sulphonate) and coated with a platelet-rich plasma protein biofilm. J Bioact Compat Polym. 2016;31(6):1–15.CrossRefGoogle Scholar
- 60.Zhai X, Zhang C, Zhao G, Stoll S, Ren F, Leng X. Antioxidant capacities of the selenium nanoparticles stabilized by chitosan. J Nanobiotechnology. 2017;15(4):1–12.Google Scholar