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PLGA submicron particles containing chlorhexidine, calcium and phosphorus inhibit Enterococcus faecalis infection and improve the microhardness of dentin

  • Wei Fan
  • Yanyun Li
  • Danfeng Liu
  • Qing Sun
  • Mengting Duan
  • Bing FanEmail author
Biomaterials Synthesis and Characterization Original Research
  • 25 Downloads
Part of the following topical collections:
  1. Biomaterials Synthesis and Characterization

Abstract

Enterococcus faecalis (E. faecalis), a Gram-positive facultative anaerobe, is reported to take responsibility for a large portion of refractory root canal infections and root canal re-infections of human teeth. Chlorhexidine is a strong bactericide against E. faecalis but cannot infiltrate into dentinal tubules. On the other hand, a common negative effect of root canal medicaments is the decrease of dentin microhardness. In this study, poly(D,L-lactic-co-glycolide) (PLGA) submicron particles were applied as delivery carriers to load and release the chlorhexidine as well as calcium and phosphorus. The release profiles, antibacterial ability against E. faecalis, infiltration ability into dentinal tubules, biocompatibility and effects on dentin microhardness of these particles were investigated. Results revealed that encapsulated chemicals could be released in a sustained manner from the particles. The particles also exhibited excellent biocompatibility on MC3T3-E1 cells and significant antimicrobial property against E. faecalis. On dentin slices, the particles could be driven into dentinal tubules by ultrasonic activiation and inhibit E. faecalis colonization. Besides, dentin slices medicated with the particles displayed an increase in microhardness. In conclusion, PLGA submicron particles carrying chlorhexidine, calcium and phosphorus could be developed into a new intra-canal disinfectant for dental treatments.

Notes

Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (Grant No. 81570969&81470732).

Compliance with ethical standards

The teeth samples used for making dentin slices were collected under the approval of ethics committee of School and Hospital of Stomatology, Wuhan University.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Wei Fan
    • 1
  • Yanyun Li
    • 1
  • Danfeng Liu
    • 1
  • Qing Sun
    • 1
  • Mengting Duan
    • 1
  • Bing Fan
    • 1
    Email author
  1. 1.The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of StomatologyWuhan UniversityWuhanPeople’s Republic of China

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