PLGA submicron particles containing chlorhexidine, calcium and phosphorus inhibit Enterococcus faecalis infection and improve the microhardness of dentin
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Enterococcus faecalis (E. faecalis), a Gram-positive facultative anaerobe, is reported to take responsibility for a large portion of refractory root canal infections and root canal re-infections of human teeth. Chlorhexidine is a strong bactericide against E. faecalis but cannot infiltrate into dentinal tubules. On the other hand, a common negative effect of root canal medicaments is the decrease of dentin microhardness. In this study, poly(D,L-lactic-co-glycolide) (PLGA) submicron particles were applied as delivery carriers to load and release the chlorhexidine as well as calcium and phosphorus. The release profiles, antibacterial ability against E. faecalis, infiltration ability into dentinal tubules, biocompatibility and effects on dentin microhardness of these particles were investigated. Results revealed that encapsulated chemicals could be released in a sustained manner from the particles. The particles also exhibited excellent biocompatibility on MC3T3-E1 cells and significant antimicrobial property against E. faecalis. On dentin slices, the particles could be driven into dentinal tubules by ultrasonic activiation and inhibit E. faecalis colonization. Besides, dentin slices medicated with the particles displayed an increase in microhardness. In conclusion, PLGA submicron particles carrying chlorhexidine, calcium and phosphorus could be developed into a new intra-canal disinfectant for dental treatments.
This study was financially supported by the National Natural Science Foundation of China (Grant No. 81570969&81470732).
Compliance with ethical standards
The teeth samples used for making dentin slices were collected under the approval of ethics committee of School and Hospital of Stomatology, Wuhan University.
Conflict of interest
The authors declare that they have no conflict of interest.
- 6.Karpiński TM, Szkaradkiewicz AK. Chlorhexidine–pharmaco-biological activity and application. Eur Rev Med Pharmacol Sci. 2015;19:1321–6.Google Scholar
- 9.Ebru-Tirali R, Bodur H, Ece G In vitro antimicrobial activity of sodium hypochlorite, chlorhexidine gluconate and octenidine dihydrochloride in elimination of microor-ganisms within dentinal tubules of primary and permanent teeth. Med Oral Patol Oral Cir Bucal 2012:e517-22.Google Scholar
- 12.Kashi TS, Eskandarion S, Esfandyari-Manesh M, Marashi SM, Samadi N, Fatemi SM, et al. Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method. Int J Nanomed. 2012;7:221–34.Google Scholar
- 23.Alcala-Alcala S, Benitez-Cardoza CG, Lima-Munoz EJ, Pinon-Segundo E, Quintanar-Guerrero D. Evaluation of a combined drug-delivery system for proteins assembled with polymeric nanoparticles and porous microspheres; characterization and protein integrity studies. Int J Pharm. 2015;489:139–47.CrossRefGoogle Scholar
- 30.Vasita R, Katti DS. Structural and functional characterization of proteins adsorbed on hydrophilized polylactide-co-glycolide microfibers. Int J Nanomed. 2012;7:61–71.Google Scholar
- 34.Alqahtani S, Simon L, Astete CE, Alayoubi A, Sylvester PW, Nazzal S, et al. Cellular uptake, antioxidant and antiproliferative activity of entrapped alpha-tocopherol and gamma-tocotrienol in poly (lactic-co-glycolic) acid (PLGA) and chitosan covered PLGA nanoparticles (PLGA-Chi). J Colloid Interface Sci. 2015;445:243–51.CrossRefGoogle Scholar
- 35.Trif M, Florian PE, Roseanu A, Moisei M, Craciunescu O, Astete CE, et al. Cytotoxicity and intracellular fate of PLGA and chitosan-coated PLGA nanoparticles in Madin-Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells. J Biomed Mater Res A. 2015;103:3599–611.CrossRefGoogle Scholar