Enhancement of bone mineral density and body mass in newborn chickens by in ovo injection of ionic-hydroxyapatite nanoparticles of bacterial origin

  • Elham Ahmadzadeh
  • Farid Talebnia RowshanEmail author
  • Mehrdad Mashkour
Engineering and Nano-engineering Approaches for Medical Devices Original Research
Part of the following topical collections:
  1. Engineering and Nano-engineering Approaches for Medical Devices


Using non-drug, non-surgical treatments for improving bone mineral diseases in newborn babies is an important topic for neonatologists. The present study introduces bacterial synthesized ionic nano-hydroxyapatite (Bio-HA) for the development of bone mineral density in the chicken embryo model. In vitro cytotoxicity analyses were demonstrated the optimal concentrations of Bio-HA compared to a chemically-synthesized hydroxyapatite (Ch-HA). Toxicity of Bio-HA on MCF-7 human cell lines was negligible at the concentrations less than 500 μg/mL whereas Ch-HA showed similar results at the concentrations less than 100 μg/mL. Therefore, concentrations at 50 μg/mL and 100 μg/mL were selected for in ovo injection of both materials into the fertilized eggs. The newly hatched chickens were sacrificed in order to monitor their serological factors, total body mass, bone mineral contents and bone mineral density. The results confirmed that Bio-HA increased the average body weight and bone mineral indices of chickens in comparison to the Ch-HA and negative controls (normal saline and intact groups). In view of the intact group, no liver or kidney damage occurred in the groups receiving Bio-HA which promises the effectiveness of these nanoparticles for the treatment of afterbirth bone mineral deficiency.



The authors wish to appreciate the valuable guidance provided by Dr Babak Mostaghaci and Faculty of Shahmirzad School of Veterinary Medicine, Semnan University, regarding animal experiments.The authors also declare that the results presented in this manuscript are entirely original, and have not been either published or under submission/review process for another journal.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of Chemical EngineeringBabol Noshirvani University of TechnologyBabolIran
  2. 2.Department of Manufacturing TechnologySanofi PasteurTorontoCanada

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