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A covalently crosslinked polysaccharide hydrogel for potential applications in drug delivery and tissue engineering

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Abstract

The development of non-cytotoxic hydrogels that can allow for the controlled release of molecules has important clinical and therapeutic applications. In this paper, we developed a series of in situ hydrogels by combining N,O-carboxymethyl chitosan and oxidized alginate without additional crosslinking agents. The rheological properties of these hydrogels as well as their gelling time, swelling ratio, and in vitro degradation behavior were investigated. We observed that although gelation was rapid at physiological temperature, it was even faster in the presence of higher oxidization degree of alginate. In vitro cytotoxicity study showed that the developed hydrogels were not cytotoxic after 24 h of culturing with NIH-3T3 cells. Additionally, bovine serum albumin was released from the hydrogels initially by diffusion at early stages followed by a degradation-dependent mechanism at later stages. In conclusion, the developed hydrogel might have potential application in the drug delivery system and tissue engineering.

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Acknowledgments

This research was supported by the Zhejiang province Foundation for Innovative Teams in Ophthalmology (Grant No. 2011R09039-12), the fund for Key Scientific and Technological Innovation in Zhejiang Province (2009R50039) and the Zhejiang Provincial Natural Science Foundation (LQ12H12003).

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Authors and Affiliations

  1. Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical College, 270 Xueyuan Road, Wenzhou, 325027, China

    Xingyi Li, Yuhua Weng, Binjun Zhang, Mei Li, Kai Diao, Zhaoliang Zhang & Hao Chen

  2. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, No. 1, Keyuan 4th Road, Chengdu, 610041, China

    Xiangye Kong

  3. Department of Lymphoma, Sino-US Center for Lymphoma and Leukemia, Tianjin Medical University Cancer Hospital and Institute, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University, Tianjin, 300060, China

    Xianhuo Wang

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  1. Xingyi Li
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  2. Yuhua Weng
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  3. Xiangye Kong
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  4. Binjun Zhang
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  5. Mei Li
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  6. Kai Diao
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  7. Zhaoliang Zhang
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  8. Xianhuo Wang
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  9. Hao Chen
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Correspondence to Hao Chen.

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Li, X., Weng, Y., Kong, X. et al. A covalently crosslinked polysaccharide hydrogel for potential applications in drug delivery and tissue engineering. J Mater Sci: Mater Med 23, 2857–2865 (2012). https://doi.org/10.1007/s10856-012-4757-5

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  • DOI: https://doi.org/10.1007/s10856-012-4757-5

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