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High-throughput-generating water-in-water droplet for monodisperse biocompatible particle synthesis

  • Qingquan Zhang
  • Jiaqu Chen
  • Hongwei GaiEmail author
Materials for life sciences
  • 19 Downloads

Abstract

Water-in-water (W/W) droplets are biocompatible vessels for bioanalysis and biomolecules delivery. Due to the ultralow interfacial tension, the stable generation of W/W droplets still faces some challenges. In this paper, we present a robust and high-throughput microfluidic platform to fabricate W/W droplets without requiring external perturbation. Using the assembled evaporation pump, W/W droplets are generated uniformly and stably for nearly 1 h. The molecular weights, concentrations, and flow rates were changed to regulate the droplet size in the range of 44–93 μm. The production of droplets is scaled up by parallelizing eight droplet-formation units on a 3-D microdevice, and the variable coefficient of droplet size in all units reaches 3.2%. Using these W/W droplets as microreactors, monodispersed hydrogel particles are synthesized by either UV light or calcium ions, and recovered conveniently without cumbersome post-processing. The established method is simple, robust and suitable for various aqueous two-phase systems, displaying its potential in biocompatible carrier synthesis.

Notes

Acknowledgements

The authors are grateful to the Natural Science Foundation of China (NSFC 21575053, 21775057), the project of Six Talent Peaks (2017SWYY-013), “333” project of Jiangsu Province, The Natural Science Foundation of the Jiangsu Higher Education Institutions of China (16KJA150006), Postgraduate Research & Practice Innovation Program of Jiangsu Province (771231814).

Supplementary material

10853_2019_4001_MOESM1_ESM.docx (2.1 mb)
Supplementary material 1 (DOCX 2132 kb)

References

  1. 1.
    Hardt S, Hahn T (2012) Microfluidics with aqueous two-phase systems. Lab Chip 12:434–442CrossRefGoogle Scholar
  2. 2.
    Song Y, Shum HC (2012) Monodisperse w/w/w double emulsion induced by phase separation. Langmuir 28:12054–12059CrossRefGoogle Scholar
  3. 3.
    Iqbal M, Tao Y, Xie S, Zhu Y, Chen D, Wang X, Huang L, Peng D, Sattar A, Shabbir MA, Hussain HI, Ahmed S, Yuan Z (2016) Aqueous two-phase system (ATPS): an overview and advances in its applications. Biol Proced Online 18:18CrossRefGoogle Scholar
  4. 4.
    Sobrinos-Sanguino M, Zorrilla S, Keang CND, Monterroso B, Rivas G (2017) Encapsulation of compartmentalized cytoplasm mimic within lipid membrane by microfluidics. Chem Commun 53:4775–4778CrossRefGoogle Scholar
  5. 5.
    Douliez JP, Martin N, Beneyton T, Eloi JC, Chapel JP, Navailles L, Baret JC, Mann S, Béven L (2018) Preparation of swellable hydrogel-containing colloidosomes from aqueous two-phase Pickering emulsion droplets. Angew Chem Int Ed 57:7780–7784CrossRefGoogle Scholar
  6. 6.
    Zhang L, Cai LH, Lienemann PS, Rossow T, Polenz I, Vallmajo-Martin Q, Ehrbar M, Na H, Mooney DJ, Weitz DA (2016) One-step microfluidic fabrication of polyelectrolyte microcapsules in aqueous conditions for protein release. Angew Chem Int Ed 55:13470–13474CrossRefGoogle Scholar
  7. 7.
    Lee TY, Ku M, Kim B, Lee S, Yang J, Kim SH (2017) Microfluidic production of biodegradable microcapsules for sustained release of hydrophilic actives. Small 13:1700646CrossRefGoogle Scholar
  8. 8.
    Navi M, Abbasi N, Jeyhani M, Gnyawali V, Tsai SSH (2018) Microfluidic diamagnetic water-in-water droplets: a biocompatible cell encapsulation and manipulation platform. Lab Chip 18:3361–3370CrossRefGoogle Scholar
  9. 9.
    Song Y, Sauret A, Shum HC (2013) All-aqueous multiphase microfluidics. Biomicrofluidics 7:061301CrossRefGoogle Scholar
  10. 10.
    Helfrich MR, El-Kouedi M, Etherton MR, Keating CD (2005) Partitioning and assembly of metal particles and their bioconjugates in aqueous two-phase systems. Langmuir 21:8478–8486CrossRefGoogle Scholar
  11. 11.
    Shum HC, Varnell J, Weitz DA (2012) Microfluidic fabrication of water-in-water (w/w) jets and emulsions. Biomicrofluidics 6:012808CrossRefGoogle Scholar
  12. 12.
    Moon BU, Jones SG, Hwang DK, Tsai SS (2015) Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures. Lab Chip 15:2437–2444CrossRefGoogle Scholar
  13. 13.
    Choi YH, Song YS, Kim DHJ (2010) Droplet-based microextraction in the aqueous two-phase system. J Chromatogr A 1217:3723–3728CrossRefGoogle Scholar
  14. 14.
    Sauret A (2012) Forced generation of simple and double emulsions in all-aqueous systems. Appl Phys Lett 100:154106CrossRefGoogle Scholar
  15. 15.
    Ziemecka I, van Steijn V, Koper GJ, Rosso M, Brizard AM, van Esch JH, Kreutzer MT (2011) Monodisperse hydrogel microspheres by forced droplet formation in aqueous two-phase systems. Lab Chip 11:620–624CrossRefGoogle Scholar
  16. 16.
    Zhou C, Zhu P, Tian Y, Tang X, Shi R, Wang L (2017) Microfluidic generation of aqueous two-phase-system (ATPS) droplets by oil-droplet choppers. Lab Chip 17:3310–3317CrossRefGoogle Scholar
  17. 17.
    Liu HT, Wang H, Wei WB, Liu H, Jiang L, Qin JH (2018) A microfluidic strategy for controllable generation of water-in-water droplets as biocompatible microcarriers. Small 14:e1801095CrossRefGoogle Scholar
  18. 18.
    Mastiani M, Seo S, Jimenez SM, Petrozzi N, Kim MM (2017) Flow regime mapping of aqueous two-phase system droplets in flow-focusing geometries. Colloid Surface A 531:111–120CrossRefGoogle Scholar
  19. 19.
    Moon BU, Abbasi N, Jones SG, Hwang DK, Tsai SS (2016) Water-in-water droplets by passive microfluidic flow focusing. Anal Chem 88:3982–3989CrossRefGoogle Scholar
  20. 20.
    Moon BU, Hwang DK, Tsai SS (2016) Shrinking, growing, and bursting: microfluidic equilibrium control of water-in-water droplets. Lab Chip 16:2601–2608CrossRefGoogle Scholar
  21. 21.
    Mastiani M, Seo S, Mosavati B, Kim M (2018) High-throughput aqueous two-phase system droplet generation by oil-free passive microfluidics. ACS Omega 3:9296–9302CrossRefGoogle Scholar
  22. 22.
    Li HL, Xue YJ, Xu M, Zhao WF, Zong CH, Liu XJ, Zhang QQ (2017) Viscosity based droplet size controlling in negative pressure driven droplets generator for large-scale particle synthesis. Electrophoresis 38:1736–1742CrossRefGoogle Scholar
  23. 23.
    Zhang QQ, Xu M, Liu XJ, Zhao WF, Zong CH, Yu Y, Wang Q, Gai HW (2016) Fabrication of Janus droplets by evaporation driven liquid–liquid phase separation. Chem Commun 52:5015–5018CrossRefGoogle Scholar
  24. 24.
    Zhang QQ, Liu XJ, Liu DY, Gai HW (2014) Ultra-small droplet generation via volatile component evaporation. Lab Chip 14:1395–1400CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Jiangsu Key Laboratory of Green Synthesis for Functional Materials, School of Chemistry and Materials ScienceJiangsu Normal UniversityXuzhouChina

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