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Journal of Materials Science

, Volume 54, Issue 13, pp 9679–9688 | Cite as

High sensitivity detection of human serum albumin using a novel magnetoelastic immunosensor

  • Rong Liu
  • Xing Guo
  • Jingzhe Wang
  • Jinyu Guo
  • Yixia Zhang
  • Wendong Zhang
  • Shengbo SangEmail author
Materials for life sciences
  • 44 Downloads

Abstract

In this paper, a novel and wireless magnetoelastic (ME) immunosensor for human serum albumin (HSA) detection is presented. Gold nanoparticles layer on the sensor surface can enhance its biocompatibility, stability and sensitivity. The anti-HSA IgG, as a capture probe, was immobilized on the gold-plated ME sensor surface to specifically recognize the target HSA. The binding complex accumulated on the immunosensor surface leads to a decrease in the immunosensor’s resonance frequency, corresponding to the HSA concentrations. The atomic force microscopy, the Raman spectrum and the X-ray electron spectroscopy were used to confirm that the antibody immobilization was successful. Furthermore, to enhance its sensitivity, the working concentration of the antibody was optimized to be 25 µg ml−1. The experimental results demonstrated that the immunosensor exhibited a linear response to the logarithm of HSA concentrations ranging from 0.01 to 100 µg ml−1, with the sensitivity of 9.3 Hz/μg ml−1 and the detection limit of 0.01 µg ml−1, which is significantly lower than the minimum diagnosis limit of urine microalbumin.

Notes

Acknowledgements

The authors are grateful for the financial support from the National Natural Science Foundation of China (Nos. 51622507, 61471255, 61474079, 61501316, 51505324).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10853_2019_3554_MOESM1_ESM.docx (548 kb)
Supplementary material 1 (DOCX 548 kb)

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education and College of Information and ComputerTaiyuan University of TechnologyJinzhongChina
  2. 2.Institute of Applied Mechanics and National Demonstration Center for Experimental Mechanics EducationCollege of Mechanical and Vehicle Engineering of Taiyuan University of TechnologyJinzhongChina

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