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Journal of Materials Science

, Volume 54, Issue 11, pp 8613–8626 | Cite as

Biotin-modified bovine serum albumin nanoparticles as a potential drug delivery system for paclitaxel

  • Danfeng Wang
  • Na LiangEmail author
  • Yoshiaki Kawashima
  • Fude Cui
  • Pengfei Yan
  • Shaoping SunEmail author
Materials for life sciences
  • 177 Downloads

Abstract

This study proposed a novel injectable nanocarrier for paclitaxel (PTX) based on biotin-modified bovine serum albumin (BSA). First, the biotin-modified BSA (biotin-BSA) was successfully synthesized and characterized by FT-IR analysis. Then, the PTX-loaded biotin-BSA nanoparticles (NPs) were prepared by disulfide bond reducing method and stabilized through the formation of intermolecular disulfide bonds. The influence of solution pH, glutathione concentration, temperature and organic solvent on the formation of NPs was studied. Under the optimized conditions, the PTX-loaded NPs had a mean diameter of 163 nm with zeta potential of − 35 mV. Transmission electron microscopy analysis showed that the NPs were sphere in shape and had a narrow size distribution. XRD and DSC spectra confirmed the successful encapsulation of PTX in the NPs in an amorphous state. The MTT assay verified that the in vitro cytotoxicity of PTX-loaded biotin-BSA NPs to biotin receptor-positive MCF-7 cells was a little higher than that of Taxol®. The cellular uptake experiments by flow cytometry demonstrated the biotin receptor-mediated endocytosis of biotin-BSA NPs. It can be concluded that the PTX-loaded biotin-BSA NPs were a promising drug delivery carrier for PTX.

Notes

Acknowledgements

The financial support from the National Natural Science Foundation of China (No. 51403057), Heilongjiang Natural Science Foundation (No. E2018052), Research and Development Project of Scientific and Technological Achievements for Colleges and Universities of Heilongjiang Province (No. TSTAU-R2018023), Harbin Science and Technology Innovation Talents Special Fund Project (Nos. 2016RQQXJ097 and 2016RQQXJ131) and the Doctoral Scientific Research Startup Foundation of Harbin Normal University (No. XKB201304) is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Chemical Engineering Process and Technology for High-Efficiency Conversion, College of Heilongjiang Province, School of Chemistry and Material ScienceHeilongjiang UniversityHarbinChina
  2. 2.Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical EngineeringHarbin Normal UniversityHarbinChina
  3. 3.Department of Pharmaceutical Engineering, School of PharmacyAichi Gakuin UniversityNagoyaJapan
  4. 4.School of PharmacyShenyang Pharmaceutical UniversityShenyangChina

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