Journal of Materials Science

, Volume 49, Issue 20, pp 7253–7262 | Cite as

Electrospinning of poly (ε-caprolactone-co-lactide)/Pluronic blended scaffolds for skin tissue engineering

  • Ning-hua Liu
  • Jian-feng Pan
  • Yue-E Miao
  • Tian-xi Liu
  • Feng Xu
  • Hui SunEmail author


For skin tissue engineering, an ideal scaffold should mimic the natural extracellular matrix of the native skin. In this study, we reported a novel elastic sub-micron fiber scaffold blending poly (ε-caprolactone-co-lactide) (PLCL) and Pluronic at different ratios by electrospinning. PLCL and Pluronic were co-electrospun with the ratio of 100/0, 99/1, 95/5, 90/10, 85/15, and 75/25. These scaffolds were evaluated in terms of fiber morphology, mechanical properties, and hydrophilicity for the purpose of culturing adipose-derived stem cells (ADSCs). Cell attachment and proliferation on the scaffolds were also evaluated to demonstrate the potential of serving as a skin graft. The results indicated that all of the electrospun fibers possessed smooth surface textures and interconnected porous structures with the average diameter ranging from approximately 750–1140 nm. The higher tensile strength was observed in 95/5 and 90/10 PLCL/Pluronic blended membranes, while further incorporation of Pluronic almost has no effect on tensile strength. The water contact angle was 85° for scaffold with the ratio of 99/1, while 0° for 90/10, 85/15, and 75/25. In addition, the elevation of Pluronic content in composition resulted in a corresponding increase in swelling behavior. Compared with PLCL, the better cell adhesion and proliferation potential of ADSCs was exhibited on all PLCL/Pluronic blended scaffolds. ADSCs on the blended scaffolds were highly elongated and well integrated with the surrounding fibers, indicating the good cytocompatibility of PLCL/Pluronic scaffolds. Thus, these blended scaffolds have the potentially high application prospect in the field of skin tissue engineering.


Pluronic Water Contact Angle Electrospun Fiber Fibrous Membrane Electrospun Scaffold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Yi-Mei Liu and Bi-Jun Zhu from biomedical research center for technical support, Dr. Zhou-Jun Wang from department of macromolecular science, and the laboratory of advanced materials for his guidance through the work, Dr. Fei Liang from Department of Statistics teaching and research for the assistance of Statistical Analysis.

Supplementary material

Supplementary material 1 (AVI 273 kb)

Supplementary material 2 (AVI 348 kb)

Supplementary material 3 (AVI 671 kb)

Supplementary material 4 (AVI 465 kb)

Supplementary material 5 (AVI 1460 kb)

Supplementary material 6 (AVI 922 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ning-hua Liu
    • 1
    • 2
  • Jian-feng Pan
    • 3
  • Yue-E Miao
    • 4
  • Tian-xi Liu
    • 4
  • Feng Xu
    • 2
  • Hui Sun
    • 1
    Email author
  1. 1.Department of Orthopaedic SurgeryShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina
  2. 2.Department of Orthopaedics SurgeryKunshan Traditional Chinese Medical HospitalSuzhouChina
  3. 3.Department of Orthopaedics Surgery, Zhongshan HospitalFudan UniversityShanghaiChina
  4. 4.State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular ScienceFudan UniversityShanghaiChina

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