Evaluation of cell proliferation and differentiation on a poly(propylene fumarate) 3D scaffold treated with functional peptides
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Synthetic polymers were used to fabricate a three-dimensional (3D) porous scaffold of poly(propylene fumarate)/diethyl fumarate (PPF/DEF). PPF-based materials are good candidates for bone regeneration, because of their non-toxic, biodegradable byproducts, and excellent mechanical properties. However, they exhibit hydrophobic surface properties that have negative effects on cell adhesion. To change the surface properties of a PPF/DEF scaffold, the authors used three peptide modifications (RGD, cyclo RGD, and RGD-KRSR mixture) to the scaffold and tested the effects on MC3T3-E1 pre-osteoblast adhesion, proliferation, and differentiation. The results indicated that peptide modification (particularly the RGD-KRDR mixture) altered the hydrophobic surface properties of the PPF/DEF scaffold, and promoted cell adhesion. Thus, it was suggest that peptide modification is a useful method for changing the properties of the PPF/DEF scaffold surface and may be applicable in bone tissue engineering.
KeywordsBone Tissue Engineering Initial Adhesion Scaffold Surface Scaffold Fabrication Peptide Modification
This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (no. 2010-0018294), the Ministry of Education, Science Technology (MEST) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Regional Innovation and WCU (World Class University) program through the Korea Science and Engineering Foundation funded by the Ministry of Education, science and Technology.