Journal of Materials Science

, Volume 46, Issue 15, pp 5260–5266 | Cite as

Method for effective immobilization of Ag nanoparticles/graphene oxide composites on single-stranded DNA modified gold electrode for enzymeless H2O2 detection

  • Wenbo Lu
  • Guohui Chang
  • Yonglan Luo
  • Fang Liao
  • Xuping SunEmail author


In this paper, we report a new method for effective immobilization of Ag nanoparticles (AgNPs) decorated graphene oxide (AgNP/GO) composites onto thiolated single-stranded DNA decorated Au electrode (AuE) surface. The novel immobilization method is based on the coordination interactions and π–π stacking interactions between DNA bases and AgNP/GO composites. The morphologies of the AgNP/GO nanocomposites are characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). It is found that the AgNP/GO-decorated AuE exhibits remarkable catalytic performance for H2O2 reduction. This H2O2 sensor has a fast amperometric response time of less than 5 s. The linear range is estimated to be from 0.1 mM to 20 mM (r = 0.998) and the detection limit is estimated to be 1.9 μM at a signal-to-noise ratio of 3, respectively.


Graphene Oxide Sodium Borohydride Adsorption Time Phosphate Buffer Saline Solution Coordination Interaction 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Wenbo Lu
    • 1
  • Guohui Chang
    • 1
  • Yonglan Luo
    • 1
  • Fang Liao
    • 1
  • Xuping Sun
    • 1
    Email author
  1. 1.Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical IndustryChina West Normal UniversityNanchongChina

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