Journal of Materials Science

, Volume 44, Issue 24, pp 6805–6810 | Cite as

Close-packed array of gold nanoparticles and sum frequency generation spectroscopy in total internal reflection: a platform for studying biomolecules and biosensors

  • Gérard TourillonEmail author
  • Laurent DreesenEmail author
  • Cédric Volcke
  • Yannick Sartenaer
  • Paul A. Thiry
  • André Peremans
Mesostructured Materials


An approach is introduced for studying the adsorption and recognition mechanisms of biomolecules, without using any markers. We show for the first time, that the sum frequency generation spectroscopy performed in the total internal reflection (TIR-SFG) geometry, combined with a regular close-packed array of gold nanoparticles allows to probe with a high sensitivity the changes in conformation and orientation induced by the recognition process of avidin by biocytin. This approach represents a new platform with potential use in biosensors, diagnostics and bioactive layers.


Gold Nanoparticles Biocytin AuNP Surface Total Internal Reflection Condition Flat Gold Surface 



L.D. and C.V. acknowledge the Walloon Region and the University of Namur—FUNDP for financial supports. C.V. is a postdoctoral researcher of the Belgian Fund for Scientific Research (F.R.S.-F.N.R.S.). This study was supported by the Centre National de la Recherche Scientifique (C.N.R.S.) and by the Belgian Fund for Scientific Research (F.R.F.C.).


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gérard Tourillon
    • 1
    Email author
  • Laurent Dreesen
    • 2
    Email author
  • Cédric Volcke
    • 2
  • Yannick Sartenaer
    • 2
  • Paul A. Thiry
    • 2
  • André Peremans
    • 2
  1. 1.Institut de Recherches sur la Catalyse et l’Environnement – IRCELYONUniversité Lyon1Villeurbanne CedexFrance
  2. 2.Research Centre in Physics of Matter and Radiation (PMR)FUNDP - University of NamurNamurBelgium

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