Self-assembly of polyaniline-grafted chitosan/glucose oxidase nanolayered films for electrochemical biosensor applications
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Chitosan (CS), an N-deacetylated derivative of chitin, is a nature occurring biopolymer found in the exoskeleton of crustaceans, in fungal cell wall, and in other biological materials . It has unusual combination properties , which includes excellent membrane-forming ability towards water, good adhesion, nontoxicity, high mechanical strength, especially good biocompatibility and susceptibility to chemical modification due to the presence of reactive amino and hydroxyl function groups. In recent years, chitosan was generally used to immobilize various enzymes. And we have reported the first attempt to prepare chitosan/glucose oxidase (GOD) nanolayered films for electrode modification by the technique of layer-by-layer (LBL) self-assembly . It was found that the response current of the CS/GOD LBL films to glucose was improved since the amount of the immobilization enzyme increased with the increase of the layer number in a certain range. However, chitosan is nearly...
KeywordsChitosan Polyaniline Phosphate Buffer Solution Graft Copolymerization Multilayer Film
We gratefully acknowledge Natural Science Foundation of Tianjin for financial support.