Journal of Materials Science

, Volume 41, Issue 15, pp 4806–4813 | Cite as

Composites of amorphous calcium phosphate and poly(hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) for bone substitution: assessment of the biocompatibility

  • Wolfgang Linhart
  • Wolfgang Lehmann
  • Michael Siedler
  • Fabian Peters
  • Arndt F. Schilling
  • Karsten Schwarz
  • Michael Amling
  • Johannes Maria Rueger
  • Matthias EppleEmail author


Composites of amorphous carbonated apatite and poly-(R)-3-hydroxybutyrate (PHB) and poly-(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate (PHBHV), respectively, were studied by pH monitoring upon immersion in water and by long-term osteoblast culture. For comparison, α-tricalcium phosphate (α-TCP), one glass ceramic (GB 9N), and one bioglass (Mg 5) were subjected to the same experiments. Excellent cell proliferation was found on the composite of calcium phosphate with PHB and on the reference materials (α-TCP, glass ceramic, bioglass). In contrast, cell death was observed repeatedly on the composite with PHBHV. A composite of amorphous calcium phosphate and PHB appears to be well suited as slowly biodegradable bone substitution material.


Osteocalcin Calcium Phosphate Glass Ceramic Amorphous Calcium Phosphate Primary Osteoblast 



We thank C. Müldner, R. Walter, D. Keyser, and D. Tadic for experimental assistance. Financial assistance by the Deutsche Forschungsgemeinschaft (Graduiertenkolleg 476 to J.M.R. and M.E.; fellowships to A.F.S., K.S. and M.S.) is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Wolfgang Linhart
    • 1
  • Wolfgang Lehmann
    • 1
  • Michael Siedler
    • 2
  • Fabian Peters
    • 2
  • Arndt F. Schilling
    • 1
  • Karsten Schwarz
    • 2
  • Michael Amling
    • 1
  • Johannes Maria Rueger
    • 1
  • Matthias Epple
    • 2
    Email author
  1. 1.Department of Trauma, Hand and Reconstructive SurgeryHamburg University, School of MedicineHamburgGermany
  2. 2.Inorganic ChemistryUniversity of Duisburg–EssenEssenGermany

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