The relevance of ANXA5 genetic variants on male fertility
To investigate the effect of the anticoagulation factor annexin A5 on male fertility and to provide perspective on the influence of members of the coagulation cascade on fertility.
Patients with normozoospermia and with unexplained severe oligozoospermia were retrospectively selected and their genomic DNA sequenced for the promoter region of ANXA5. The genotypes proportions and the odds ratio for carriership of the haplotype M2 were compared between the groups and population control. The clinical data used were gathered from parameters determined during routine clinical assessment and were compared between carriers and non-carriers within the patient groups.
The carrier rates for the haplotype M2/ANXA5 were of 25.73%, 20.81%, and 15.3% in the severe oligozoospermic, the normozoospermic, and the general population control groups, respectively. The OR between patients groups was of 1.31 (95% CI 0.88 to 1.96 p = 0.176). Oligozoospermic and normozoospermic patients compared with the control group had an OR of 1.9 (95% CI 1.33 to 2.73 p < 0.001) and 1.45 (95% CI 0.99 to 2.10 p = 0.054) respectively. The clinical parameters that differed between the carriers and non-carriers of the haplotype M2/ANXA5 were prolactin, α-glucosidase, and fructose. The differences were only statistically significant in the normozoospermic group.
Athough the infertile patient groups had a higher prevalence of promoter variants, we could not demonstrate any biologically relevant effect of lower levels of annexin A5 on most male fertility parameters. A deficiency in an anticoagulation factor does not seem to impact male fertility.
KeywordsAnnexin A5 Male fertility Haplotype M2 ANXA5 Sperm count Coagulation cascade
The authors thank Michelina Kierzek for language editing the manuscript and Albrecht Röpke for the laboratory work.
Full doctorate fellowship for Heloisa Lopes Lavorato was provided by the Brazilian program Science without Borders by the Brazilian funding agency Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) under the process 206742/2014-2. A STIBET-DAAD fellowship was awarded to HL by the University Münster. Experimental work was supported by the DFG Clinical Research Unit 326.
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