Journal of Assisted Reproduction and Genetics

, Volume 35, Issue 9, pp 1573–1583 | Cite as

Euploid embryos selected by an automated time-lapse system have superior SET outcomes than selected solely by conventional morphology assessment

  • E. Rocafort
  • M. Enciso
  • A. Leza
  • J. Sarasa
  • J. Aizpurua
Assisted Reproduction Technologies



We investigated if automated TLI selection may be a valuable strategy to identify those euploid embryos with the best chances of success.


This is a unicentric and retrospective study involving 244 patients undergoing preimplantational genetic screening (PGS) cycles with autologous oocytes or oocyte donation (OD) with single euploid embryo transferred. We examined euploid embryos selected for transfer based on morphology evaluation alone (PGS-only; control group) or by assessment using an automated TLI system (Eeva™; PGS-TLI group).


In both, autologous oocytes and OD patients, significantly better implantation and clinical and ongoing pregnancy rates were obtained in the PGS-TLI group when euploid embryos with high implantation potential as predicted by the automated TLI System (Eeva™) were transferred compared with the PGS-only group. This improvement was also observed when only transfers of good morphological quality embryos were compared. TLI categories showed significant differences on blastocyst formation and euploidy rate.


Automated TLI combined with PGS is a useful prognostic tool to identify euploid embryos with the highest potential for implantation and pregnancy. Further, these results provide evidence that a healthy pregnancy does not only depend upon normal chromosomal status.


Preimplantation genetic screening Automated time-lapse imaging In vitro fertilization Oocyte donation Pregnancy rates 



The authors thank all the IVF-Spain team, especially the IVF Lab for their work and support. Medical writing assistance in the form of language editing and publication structure was provided by Kaedy Bryson (Zoetic Science, an Ashfield company, part of UDG Healthcare plc, Macclesfield, UK).

Authors’ contributions

E.R. is responsible for conception, design, acquisition, analysis, and interpretation of data and drafting and critical review of the article. M.E. is responsible for analysis and interpretation of data, drafting, and critical review of the article. A.L. is responsible for recruitment and acquisition of data. J.S. is responsible for analysis and interpretation of the data and critical review of the article. J.A. is responsible for the critical review of the article.


No external funding was either sought or obtained for this study.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Supplementary material

10815_2018_1265_MOESM1_ESM.docx (73 kb)
ESM 1 (DOCX 73 kb)
10815_2018_1265_MOESM2_ESM.docx (84 kb)
ESM 2 (DOCX 83 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.IVF LaboratoryIVF SpainAlicanteSpain
  2. 2.BarcelonaSpain
  3. 3.Genetics DepartmentiGLSAlicanteSpain
  4. 4.Reproductive MedicineIVF SpainAlicanteSpain

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