Journal of Applied Spectroscopy

, Volume 85, Issue 6, pp 1064–1070 | Cite as

Molecular Spectroscopy Evidence of Effective Constituents in the Binding of Angelica sinensis to Bovine Serum Albumin: Comparative Binding and Thermodynamic Profile

  • Zhenzhong Wang
  • Xuehua Deng
  • Jia Hou
  • Jing FanEmail author

Some bio-effective constituents, such as organic acids and phthalides, from the root of Angelica sinensis can be observed in the blood after oral intake. In this work, two organic acids, ferulic acid and anisic acid, and two phthalides, senkyunolide I and butylidenephthalide, were selected to investigate the interaction with bovine serum albumin systematically, using a fluorescence spectroscopic method under physiological conditions. The Stern–Volmer quenching constant, binding constant, and the corresponding thermodynamic parameters and binding numbers were measured. Synchronous fluorescence and three-dimensional fluorescence spectroscopy were also used. The results provided molecular spectroscopy evidence that some constituents of Angelica sinensis are absorbed and transported into the blood.


Angelica sinensis fluorescence bovine serum albumin ferulic acid butylidenephthalide senkyunolide I 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhenzhong Wang
    • 1
    • 2
    • 3
  • Xuehua Deng
    • 1
    • 2
    • 3
  • Jia Hou
    • 4
  • Jing Fan
    • 1
    • 2
    • 3
    Email author
  1. 1.Pharmacy College of Hubei University of MedicineShiyanChina
  2. 2.Key Lab of Wudang Characteristic Herbal Study in Hubei ProvinceShiyanChina
  3. 3.Engineering Research Center for Herbal Utilization of Hubei ProvinceShiyanChina
  4. 4.Gansu University of Chinese MedicineLanzhouChina

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