Monthly variations of fucoidan content and its composition in the farmed brown alga Saccharina sculpera (Laminariales, Phaeophyceae)
Crude fucoidan was extracted from the Saccharina sculpera from Rongcheng cultivation area in Sanggou Bay in the Yellow Sea of China which was sampled monthly from March to July. The crude fucoidan yield of S. sculpera showed an increase from 2.9% in March to 6.1% dry wt in July as the plant matured. And the content of polysaccharide (from 0.55 to 1.46% dry wt) and sulfate (from 0.37 to 1.62% dry wt) tended to increase from March to July as well. The monosaccharide composition changed constantly in the growing period with an increase of fucose (from 32.15 to 60.85 mol%) and a decrease of galactose (from 21.89 to 9.03 mol%). Analysis results showed that the content of crude fucoidan, polysaccharide, and sulfate in matured season was higher than that of other three species of farmed brown algae Saccharina japonica, Costaria costata, and Undaria pinnatifida in the same waters. These data indicated that S. sculpera was an excellent algal species in fucoidan content. This study on seasonal variations of polysaccharide, sulfate content, and monosaccharide composition provides a practical guidance for determining the optimal opportunity for harvesting the algae in order to standardize preparations of fucoidan for use in the pharmaceutical industry.
KeywordsSaccharina sculpera Phaephyceae Fucoidan content Monthly variations Polysaccharide Sulfate Monosaccharide composition
We wish to thank the anonymous reviewers for their valuable comments on this manuscript. We wish to thank Professor Li Sun for his kind help in preparing the manuscript.
This study was supported by China Agriculture Research System (CARS-50), the Special Funds for Key Laboratories Construction of Shandong Province (SDKL2017009), the Developmental Program of Science and Technology of Yantai (2018LJRC067), and the Special Funds for Modern Agricultural Industry Technology System Construction of Shandong Province (SDAIT-26-11).
- Ai O, Mizuta H, Yamamoto H (2011) Physiological differences between the nutrient uptakes of Kejellmaniella crassifolia and Laminaria japonica (Phaeophyceae). Fish Sci 67:415–419Google Scholar
- Bartsch I, Wiencke C, Bischof K, Buchholz CM, Buck BH, Eggert A, Feuerpfeil P, Hanelt D, Jacobsen S, Karez R, Karsten U, Molis M, Roleda MY, Schubert H, Schumann R, Valentin K, Weinberger F, Wiese J (2008) The genus Laminaria sensu lato: recent insights and developments. Eur J Phycol 43:1–86CrossRefGoogle Scholar
- Bruhn A, Janicek T, Manns D, Nielsen MM, Balsby TJS, Meyer AS, Rasmussen MB, Hou X, Saake B, Göke C, Bjerre AB (2017) Crude fucoidan content in two North Atlantic kelp species, Saccharina latissima and Laminaria digitata—seasonal variation and impact of environmental factors. J Appl Phycol 29:3121–3137CrossRefGoogle Scholar
- Chen Q, Ma TX, Guo HJ (2012) Determination of the sulfate content in sulfated fucans by barium sulfate turbidity. J Pharm Pract 30:118–120Google Scholar
- Deslandes E, Pondaven P, Auperin T, Roussakis CC, Guézennec J, Stiger V, Payri C (2000) Preliminary study of the in vitro antiproliferative effect of a hydroethanolic extract from the subtropical seaweed Turbinaria ornata (Turner) J. Agardh on a human non-small-cell bronchopulmonary carcinoma line (NSCLC-N6). J Appl Phycol 12:257–262CrossRefGoogle Scholar
- He YH, Wang QK, Liu HD (2006) Extracting technology of fucoidan from kelp Laminaria japonica by enzymatic hydrolysis. J Dalian Fisheries Univ 21:55–58Google Scholar
- Holland R (2011) Effect of oil spills on the early development of ecologically important rocky shore seaweeds can we use them as indicators of spill impact. Proc Int Oil Spill Conf 1:137Google Scholar
- Percival E, McDowell RH (1967) Chemistry and enzymology of marine algal polysaccharides. Carbohydr Res 11:582Google Scholar
- Toth R (1974) Sporangial structure and zoosporogenesis in Chorda tomentosa (Laminariales). J Phycol 10:170–185Google Scholar
- Xing LH, Zhu CJ, Sun WH (2011) Determination of monosaccharides and uronic acids of fucoidan by high performance liquid chromatography with pre-column derivatization. Chinese Fishery Quality and Standards 1:64–69Google Scholar
- Zhang QB, Xu ZH (1996) Advances in the chemistry of fucoidan. Chin J Mar Drugs 15:38–41Google Scholar