Industrial production of Phaeodactylum tricornutum for CO2 mitigation: biomass productivity and photosynthetic efficiency using photobioreactors of different volumes
The photosynthetic efficiency (PE) and potential of Phaeodactylum tricornutum for CO2 mitigation in industrial tubular photobioreactors (PBRs) of different volumes were evaluated. A preliminary assay was performed at lab-scale to optimize the salt concentration of the culture medium. Interestingly, salinity did not affect the growth of P. tricornutum at concentrations of 2.5, 5, 10, and 20 g L−1. Higher volumetric productivities were achieved in the 2.5-m3 tubular PBR (0.235 g L−1 day−1), followed by 35- and 10-m3 PBRs. Maximum areal productivities corresponded to 48.5, 45.0, and 12.8 g m−2 day−1 for the 35-, 10-, and 2.5-m3 PBRs, respectively. PE was thus higher in the 35- and 10-m3 PBRs (2.21 and 2.08%, respectively). The 10- and 35-m3 PBR showed CO2 mitigation efficiencies of 60 and 41%, respectively, of the CO2 introduced into the PBR, corresponding to 2.3 and 2.5 g of fixed CO2 per g of biomass. Overall, cultivation of P. tricornutum couples high PE and areal productivity when the industrial PBRs were used, particularly PBRs of larger volumes. This improved PE performance with larger PBR volumes strongly suggests that large-scale cultivation of this diatom holds great potential for industrial CO2 mitigation.
KeywordsMicroalgae CO2 mitigation Photobioreactors Phaeodactylum tricornutum Industrial production
PQ conceived, designed, and performed the experiments, interpreted the data with statistical expertise, prepared the figures/tables, and wrote the manuscript; MT, JTS, AM, and TS collected and assembled the data, prepared figures/tables, and drafted the manuscript; HP and JV designed the experiences, analyzed and interpreted the data, and critically revised the manuscript; MS and JLS designed the experiences, analyzed and interpreted the data, obtaining of funding or logistic support, and critically revised the manuscript. All authors have read the manuscript and approved this submission.
The present work was funded by the Portuguese national budget P2020 in the scope of the project no. 023310 – ALGACO2: “Cultivo industrial de microalgas como tecnologia verde para captura de CO2 atmosférico” and CCMAR/Multi/04326/2013 grant of the Foundation for Science and Technology (FCT). H.P. (SFRH/BD/105541/2014) was funded by a PhD grant from FCT.
- Bowler C, Allen AE, Badger JH, Grimwood J, Jabbari K, Kuo A, Maheswari U, Martens C, Maumus F, Otillar RP, Rayko E, Salamov A, Vandepoele K, Beszteri B, Gruber A, Heijde M, Katinka M, Mock T, Valentin K, Verret F, Berges JA, Brownlee C, Cadoret JP, Chiovitti A, Choi CJ, Coesel S, de Martino A, Detter JC, Durkin C, Falciatore A, Fournet J, Haruta M, Huysman MJJ, Jenkins BD, Jiroutova K, Jorgensen RE, Joubert Y, Kaplan A, Kröger N, Kroth PG, la Roche J, Lindquist E, Lommer M, Martin–Jézéquel V, Lopez PJ, Lucas S, Mangogna M, McGinnis K, Medlin LK, Montsant A, Secq MPO–L, Napoli C, Obornik M, Parker MS, Petit JL, Porcel BM, Poulsen N, Robison M, Rychlewski L, Rynearson TA, Schmutz J, Shapiro H, Siaut M, Stanley M, Sussman MR, Taylor AR, Vardi A, von Dassow P, Vyverman W, Willis A, Wyrwicz LS, Rokhsar DS, Weissenbach J, Armbrust EV, Green BR, van de Peer Y, Grigoriev IV (2008) The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature 456:239–244PubMedCrossRefGoogle Scholar
- Dragone G, Fernandes B, Vicente AA, Teixeira JA (2010) Third generation biofuels from microalgae. In: Mendez-Vilas A (ed) Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 1st edn. Formatex Research Center, Badajoz, pp 1355–1366Google Scholar
- Marinho YF, Brito LO, Campos CVS, Severi W, Andrade HA, Galvez AO (2017) Effect of the addition of Chaetoceros calcitrans, Navicula sp. and Phaeodactylum tricornutum (diatoms) on phytoplankton composition and growth of Litopenaeus vannamei (Boone) postlarvae reared in a biofloc system. Aquac Res 48:4155–4164CrossRefGoogle Scholar
- Morais KCC, Vargas JVC, Mariano AB, Ordonez JC, Kava V (2016) Sustainable energy via biodiesel production from autotrophic and mixotrophic growth of the microalga Phaeodactylum tricornutum in compact photobioreactors. SusTechGoogle Scholar
- Pereira H, Gangadhar KN, Schulze PSC, Santos T, Sousa CB, Schueler LM, Custódio L, Malcata FX, Gouveia L, Varela JCS, Barreira L (2016) Isolation of a euryhaline microalgal strain, Tetraselmis sp. CTP4, as a robust feedstock for biodiesel production. Sci Rep 6:35663PubMedPubMedCentralCrossRefGoogle Scholar
- Pereira H, Páramo J, Silva J, Marques A, Barros A, Maurício D, Santos T, Schulze P, Barros R, Gouveia L, Barreira L, Varela J (2018) Scale-up and large-scale production of Tetraselmis sp. CTP4 (Chlorophyta) for CO2 mitigation: from an agar plate to 100-m3 industrial photobioreactors. Sci Rep 8:5112PubMedPubMedCentralCrossRefGoogle Scholar
- Waters CN, Zalasiewicz J, Summerhayes C, Barnosky AD, Poirier C, Gałuszka A, Cearreta A, Edgeworth M, Ellis EC, Ellis M, Jeandel C, Leinfelder R, McNeill JR, Richter D, Steffen W, Syvitski J, Vidas D, Wagreich M, Williams M, Zhisheng A, Grinevald J, Odada E, Oreskes N, Wolfe AP (2016) The Anthropocene is functionally and stratigraphically distinct from the Holocene. Science 351:137–148CrossRefGoogle Scholar