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Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater

  • Natanamurugaraj Govindan
  • Gaanty Pragas Maniam
  • Mashitah M. Yusoff
  • Mohd Hasbi Ab. Rahim
  • Tawan Chatsungnoen
  • Rameshprabu Ramaraj
  • Yusuf ChistiEmail author
Article

Abstract

The marine diatom Gyrosigma sp. was cultured in a medium comprised of inorganic nutrients dissolved in palm oil mill effluent (POME) wastewater. The production of lipids in the biomass was optimized using a statistical design of experiments in combination with the response surface method. The experimental factors were incident light level and initial concentrations of nitrate, phosphate, and silicate in the medium in batch culture. At 25 ± 2 °C, the maximum lipid content in the biomass harvested at the end of a 12-day batch culture was 70.7 ± 6.0% by dry weight for the following values of the experimental factors: an incident light level of 131 μmol photons m−2 s−1, a nitrate concentration of 1.8 mg L−1 (29.0 μM), a phosphate concentration of 6.8 mg L−1 (71.6 μM), and a silicate concentration of 10.1 mg L−1 (132.7 μM). Under the optimized conditions, the maximum dry mass concentration of the diatom was 560 mg L−1 on day 8 of a batch culture, declining to ~409 mg L−1 on day 12. For the 12-day batch operation, the final average productivities of the biomass and the lipids were 34.1 ± 5.5 mg L−1 day−1 and 24.1 ± 0.2 mg L−1 day−1, respectively. The fatty acids in the algal lipids were found to be as follows (%, w/w of total lipids): palmitic acid (48.6%), eicosapentaenoic acid (10.6%), myristic acid (8.1%), stearic acid (8.0%), linoleic acid (7.5%), oleic acid (6.4%), and linolenic acid (5.8%). The response surface model predicted the lipid content in the biomass with a high degree of confidence.

Keywords

Bacillariophyceae Gyrosigma sp. Marine diatom Industrial wastewater Statistical optimization Eicosapentaenoic acid 

Notes

Acknowledgments

The Central Laboratory of UMP is acknowledged for allowing access to SEM and GCMS instruments and providing technical assistance with sample preparation and analyses. Stat-Ease, Inc., Minneapolis, USA, is thanked for providing the statistics software used.

Funding information

The authors gratefully acknowledge Universiti Malaysia Pahang (UMP) for financial support through the Internal Research Grant No. RDU1703167 and Flagship Grant No. RDU182205.

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences & TechnologyUniversiti Malaysia PahangKuantanMalaysia
  2. 2.Earth Resources & Sustainability CentreUniversiti Malaysia PahangKuantanMalaysia
  3. 3.Department of BiotechnologyMaejo University-Phrae CampusPhrae 54140Thailand
  4. 4.School of Renewable EnergyMaejo UniversityChiang Mai 50290Thailand
  5. 5.School of EngineeringMassey UniversityPalmerston NorthNew Zealand

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