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Effects of pH on growth and biochemical responses in Agarophyton vermiculophyllum under different temperature conditions

  • Palas Samanta
  • Sojin Jang
  • Sookkyung Shin
  • Jang Kyun KimEmail author
Article

Abstract

The effects of pH (6.2, 7.2, 8.2, 9.2, and 10.2) under rising temperature (30 °C vs 20 °C) on Agarophyton vermiculophyllum growth and bio-physiology were investigated. Results showed that A. vermiculophyllum exhibited lower growth rates under elevated temperature in all pH values. Chlorophyll a, carotenoid, and phycocyanin levels were significantly enhanced by temperature elevation (p < 0.05). Enhanced H2O2 production either at lower or higher pH values correlated with lipid peroxidation (LPO) levels under elevated temperature, which suggested oxidative stress development. Oxidative damage was more severe at elevated pH values, which is confirmed by higher reactive oxygen species (ROS) levels. Compared with ambient pH 8.2 value, lower pH values under elevated temperature lead to increase activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST), indicating that these enzymes played an important role to combat stress. However, decreased glutathione reductase (GR) and glutathione peroxidase (GPx) activities indicate least contribution for ROS scavenging at lower pH values. On contrary, SOD and CAT declined at elevated pH values compared with ambient pH, suggesting least contribution for ROS removal. Moreover, enhanced GR and GPx activities at elevated pH and temperature are not enough to scavenge ROS production. These data are consistent with higher H2O2 and LPO levels, and lower GST activities. Collectively, our results indicated that either pH fluctuations or elevated temperature displayed a disadvantageous influence on growth and bio-physiology of A. vermiculophyllum. Therefore, rising temperature alleviates adverse effects of seawater acidification, but it aggravates the negative effects of seawater alkalization on growth and bio-physiology of A. vermiculophyllum.

Keywords

Agarophyton vermiculophyllum Rhodophyta Antioxidant enzymes pH Temperature Oxidative stress 

Notes

Author contribution

All authors have approved the final article and have participated in the research and/or article preparation.

Funding information

This study was supported by Basic Science Research Programs through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1A6A1A06015181) and by the Ministry of Science and ICT (2019R1F1A1059663).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Marine ScienceIncheon National UniversityIncheonRepublic of Korea
  2. 2.Research Institute of Basic SciencesIncheon National UniversityIncheonRepublic of Korea
  3. 3.System Toxicology Research CenterKorea Institute of ToxicologyDaejeonRepublic of Korea

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