Stoichiometrically balanced nutrient management using a newly designed nutrient medium for large scale cultivation of Cyanobacterium aponinum
Cyanobacteria have tremendous potential to produce bioactive molecules, which makes them a highly lucrative organism for use in industrial applications. In the present study, the first commercial nutrient medium for Cyanobacterium aponinum is developed and a process for large scale cultivation of cyanobacteria is established by combining economical medium, pre-emptive nutrient feeding strategy, and semi-continuous mode (SCM) of cultivation. The parameters measured were growth in terms of OD750 biomass, total nitrogen, nitrate, and phosphorous. Results indicated 13% more biomass yield in urea-phosphoric acid medium (UPA), in comparison to blue-green medium (BG-11). Biomass concentration was 5.3 and 4.7 g L−1 with UPA and BG-11 media, respectively. Urea was found to be a preferred nitrogen source for C. aponinum. Nutrient-dosing studies with UPA medium in SCM of operation resulted in an average daily biomass productivity of ~ 0.44 g L−1 day−1, which is significantly higher than those reported in previous studies. Here, the stoichiometric requirement of nitrogen and phosphorous was found to be 31 mg L−1 and 4.5 mg L−1, respectively. Stoichiometric nutrient addition in SCM resulted in a reduction in nutrient loss in blow down. In addition, the outdoor scale-up studies in flat panel photobioreactors further established the efficacy of UPA medium. Cost analysis of media revealed that UPA medium is 4.4 times less expensive than BG-11 and hence is a suitable and economical medium for large scale cultivation of C. aponinum. Further, this nutrient feeding strategy has wider applications which can be extended to other algal strains and cultivation systems.
KeywordsBiomass CO2 mitigation Cyanobacterium aponinum Growth medium Photobioreactor Urea Cultivation Biofuel Algae
We sincerely acknowledge Reliance Industries Limited for providing the laboratory resources. We also appreciate Ajit Sapre (Group President, Research & Technology, Reliance Industries Limited) for his support; Saranya Karuppasamy for strain isolation and purification; Vinod Nagle and Akshay Chawande for maintaining Cyanobacterium aponinum; Badrish Soni for providing strain information; Chaitanya Joshi, Rakhi Dixit, and Ashish Waghmare for technical assistance; Debanjan Sanyal and Nishant Saxena for providing sea water analysis data and Uma Shankar Sagaram, G Venkata Subhash, and Tomal Dattaroy for their critical inputs in refining the manuscript.
This study received funding from Reliance Industries Limited.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts.
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