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Purification and characterization of fucose-containing sulphated polysaccharides from Sargassum tenerrimum and their biological activity

  • Manoj Saravana Guru Mohan
  • Anant AcharyEmail author
  • Vasanthi Mani
  • Eduardas Cicinskas
  • Aleksandra A. Kalitnik
  • Maksim Khotimchenko
Article

Abstract

The crude sulphated polysaccharides (ST-D) were extracted from Sargassum tenerrimum and purified using DEAE-Sepharose anion-exchange chromatography and yielded three fractions (ST-DFI 46.2% (w/w), ST-DFII 51.6% (w/w) and ST-DFIII 2.2% (w/w)). The monosaccharide composition analysis showed fucose (40–43%) as the major sugar. The apparent molecular weight of ST-DF1, ST-DFII and ST-DFIII was found to be 233, 286 and 88 kDa, respectively. Structural investigation analysis by FTIR and NMR revealed the presence of sulphated polysaccharides with (1,3)-linked fucose with sulphation at C4 and C2 position. The ST-DFIII was the most biologically active fraction. It had the highest anticoagulant (APTT 265 ± 2.7 s) and radical scavenging activity (superoxide radical IC50 26.1 ± 0.15 μg mL−1). ST-DFII was slightly less active than ST-DFIII but could be obtained in higher quantity. These results suggest that ST-DFII from S. tenerrimum could become a promising entity as nutraceutical molecule.

Keywords

Antioxidant Anticoagulant Fucoidan Phaeophyceae Molecular weight FRAP and ABTS 

Notes

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Center for Research, Department of BiotechnologyKamaraj College of Engineering and TechnologyMadurai,India
  2. 2.School of BiomedicineFar Eastern Federal UniversityVladivostokRussia

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