Simple method for preserving large quantity of high viability red biomass of Haematococcus in the medium term (3~6 months)
To preserve large quantity of biomass in high viability for medium-term time (3~6 months) is meaningful for large-scale cultivation of Haematococcus pluvialis. In this research, 8 preserving methods were designed and tested under room temperature (RT) as well as under sub-RT (10 °C). Results showed that the viability index for biomass that mixed with ten times volume of quartz sand and was preserved in dim light and sub-RT for 8 months was 67%, much higher than that of other methods. Other merits including simple operation, cheap and recyclable additive, and environmental friendly were also inherent characters for this method, indicating it was suitable for preserving kilogram level of biomass in high viability up to several months.
KeywordsPreservation Haematococcus pluvialis Red biomass Viability Quartz sand
This work was supported by the National Key Research and Development Program of China (grant number 2016YFB0601002) and the National Natural Science Foundation of China (31572637).