Co-cultivation and stepwise cultivation of Chaetoceros muelleri and Amphora sp. for fucoxanthin production under gradual salinity increase
In a seawater-based open pond microalgae cultivation system salinity will increase gradually over time due to evaporative loss. Continuous salinity increase would lead to non-optimal salinities which negatively affect the biomass and fucoxanthin productivity. To increase and maintain high overall biomass and fucoxanthin productivity, even in the non-optimal salinity zone, two cultivation methods for marine and halotolerant microalgae were carried out, co-cultivation and stepwise cultivation (sequential cultivation). Two fucoxanthin-producing diatoms, Chaetoceros muelleri (marine) and Amphora sp. (halotolerant), were cultivated at non-optimal salinities between 59 and 65‰. Stepwise cultivation showed approximately 63% higher total biomass and 47% higher fucoxanthin productivity than that of co-culture. The ability to reutilize culture media in the stepwise cultivation increases the sustainability of that method. The use of a stepwise culture regime, coupled with a regimen of gradually increasing salinity, provides the possibility of year round fucoxanthin production from microalgae.
KeywordsBacillariophyta Fucoxanthin Microalgae Salinity Co-cultivation Stepwise cultivation
We are thankful to Emeka G. Nwoba, PhD student, Algae R& D Centre, School of Veterinary and Life Sciences, Murdoch University, Western Australia, for his assistance in extracting fucoxanthin and lipid.
- Cosgrove JJ, Borowitzka MA (2010) Chlorophyll fluorescence terminology: an introduction. In: Suggett DJ, Borowitzka MA, Prášil O (eds) Chlorophyll a fluorescence in aquatic sciences: methods and applications. Springer, Dordrecht, pp 1–17Google Scholar
- Fon Sing S (2010) Strain selection and outdoor cultivation of halophilic microalgae with potential for large-scale biodiesel production. PhD Thesis, Murdoch University, Western Australia 221 ppGoogle Scholar
- Indrayani I (2017) Isolation and characterization of microalgae with commercial potential. PhD Thesis, Murdoch University, Western Australia 214 ppGoogle Scholar
- Zarekarizi A, Hoffmann L, Burritt D (2018) Approaches for the sustainable production of fucoxanthin, a xanthophyll with potential health benefits. J Appl Phycol. https://doi.org/10.1007/s10811-018-1558-3