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Photosynthetic activity of Prorocentrum donghaiense Lu acclimated to phosphorus limitation and its photosynthetic responses to nutrient depletion

  • Kai-Ming Sun
  • Ming Xin
  • Ping SunEmail author
  • Yan Li
  • Ruixiang Li
  • Xuexi Tang
  • Zongling Wang
Article

Abstract

Prorocentrum donghaiense blooms periodically occur in late spring and early summer in the phosphorus (P)-limited East China Sea; however, few studies have been conducted in nutrient conditions that resembled those in the East China Sea. To study the actual photosynthetic status of P. donghaiense in P-limited conditions, we established stable P-limited conditions using semi-continuous culture at two dilution rates (0.1 and 0.3 day−1). When the external nutrient concentrations remained steady after 26 days, time did not affect the maximum quantum yield (Fv/Fm), normalized Stern-Volmer quenching (NPQNSV), maximum light use efficiency (α), maximum relative electron transport rate (rETRmax), and light saturation intensity (Ik). Prorocentrum donghaiense acclimated to P limitation, and the high actual quantum yield of photosystem II (ΦPSII) and rETRmax, low NPQNSV at 0.3 day−1, and increased ΦPSII with time at 0.3 day−1 indicated the photosynthetic advantages of P. donghaiense at a high growth rate. To test the responses of P-limitation-acclimated P. donghaiense to nutrient inputs, nutrient-addition experiments were conducted. Nitrate and phosphate addition thoroughly depleted P or nitrogen (N). Greater decreases in Fv/Fm, ΦPSII, α and rETRmax, and higher NPQNSV derived from rapid light curves (RLCs) were found under the P-depletion condition than under the N depletion condition. These results showed that the P-limitation-acclimated P. donghaiense had a weaker photosynthetic tolerance to P depletion. Given the differences in the photosynthetic tolerance of P. donghaiense to nutrient depletion, our results suggested that reduced P inputs should be considered during the management of P. donghaiense blooms.

Keywords

Prorocentrum donghaiense Phosphorus limitation Nutrient depletion Fv/Fm Non-photochemical quenching Rapid light curves 

Notes

Acknowledgements

We thank two anonymous reviewers for their helpful comments. We would like to express our utmost appreciation and respect to the late Prof Mingyuan Zhu in the First Institute of Oceanography, State Oceanic Administration (SOA) for his valuable suggestions on the semi-continuous algal culture. This work was supported by the National Natural Science Foundation of China (No. 41506191).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Kai-Ming Sun
    • 1
    • 2
    • 3
  • Ming Xin
    • 1
  • Ping Sun
    • 1
    • 2
    • 4
    Email author
  • Yan Li
    • 1
  • Ruixiang Li
    • 1
  • Xuexi Tang
    • 3
  • Zongling Wang
    • 1
  1. 1.First Institute of OceanographyMinistry of Natural ResourcesQingdaoPeople’s Republic of China
  2. 2.Laboratory of Marine Ecology and Environmental ScienceQingdao National Laboratory for Marine Science and TechnologyQingdaoPeople’s Republic of China
  3. 3.College of Marine LifeOcean University of ChinaQingdaoPeople’s Republic of China
  4. 4.College of Environmental Science and EngineeringOcean University of ChinaQingdaoPeople’s Republic of China

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