Advertisement

Journal of Applied Phycology

, Volume 31, Issue 2, pp 951–958 | Cite as

Gelidium brasiliense sp. nov. (Gelidiales, Rhodophyta): a diminutive agarophyte from Brazil

  • Beatriz BrunelliEmail author
  • Mayra Jamas
  • Daniela Milstein
  • Sung M. Boo
  • Mutue T. Fujii
VI REDEALGAS WORKSHOP (RIO DE JANEIRO, BRAZIL)

Abstract

An ongoing biodiversity survey along the Brazilian coast revealed a previously unknown species of Gelidium. Morphological studies coupled with molecular phylogenetic analyses, using the molecular markers rbcL and cox1, confirmed that this is a new species of red algae, which is described and illustrated here as Gelidium brasiliense. This species is extremely small and has been found growing epiphytically on the thalli of the green alga Udotea flabellum, in the reef environments in Espírito Santo, Brazil, or epilitically in northeastern Brazil.

Keywords

Rhodophyta Biodiversity CoxGelidiaceae rbcTaxonomy 

Notes

Acknowledgments

Sincere thanks are due to Dr. Wilson Freshwater and an anonymous reviewer for review and improvement of the manuscript.

Funding information

This work was partially supported by research grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Proc. 2012/18775-6, 2016/50370-7) and from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. 484647/2012-1, 440523/2015-0). BB would also like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the Master’s dissertation fellowship. The Prof. Sung Min Boo for the support received at the Center of Seaweed Biomass in Chungnam National University, at Daejeon, Korea, during my stay, and the Cíntia Iha for supplying his DNA samples. MTF thanks CNPq for the productivity fellowship (Proc. 304899/2017-8).

References

  1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefGoogle Scholar
  2. Armisén R, Galatas F (2009) Agar. In: Phillips GO, Williams PA (eds) Hanbook of Hydrocolloids. 2nd Edn. Woodhead Publishing, Cambridge, pp 82–107Google Scholar
  3. Boo GH, Kim KM, Nelson WA, Riosmena-Rodríguez R, Yoon KJ, Boo SM (2014) Taxonomy and distribution of selected species of the agarophyte genus Gelidium (Gelidiales, Rhodophyta). J Appl Phycol 26:1243–1251CrossRefGoogle Scholar
  4. Boo GH, Le Gall L, Miller KA, Freshwater DW, Wernberg T, Terada R, Yoon KJ, Boo SM (2016a) A novel phylogeny of the Gelidiales (Rhodophyta) based on five genes including the nuclear CesA, with descriptions of Orthogonacladia gen. nov. and Orthogonacladiaceae fam. nov. Molec Phylogenet Evol 101:359–372CrossRefGoogle Scholar
  5. Boo GH, Cai Y, Boo SM (2016b) Molecular identification of gelidioid algae (Gelidiales, Rhodophyta) from Singapore with a description of Gelidium sentosaense sp. nov. Phycologia 55:247–256CrossRefGoogle Scholar
  6. Brunelli B (2017) Filogeografia de Gelidium floridanum e Pterocladiella capillacea (Gelidiales, Rhodophyta) e espécies relacionadas no Atlântico ocidental, com ênfase no Brasil, com base em dados morfológicos e moleculares. Instituto de Botânica, São Paulo 71 ppGoogle Scholar
  7. Dariba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Meth 9:772CrossRefGoogle Scholar
  8. Feldmann J, Hamel G (1936) Floridées de France. VII: Gélidiales. Rev Algol 9:85–140Google Scholar
  9. Fredriksen S, Guiry MD, Rueness J (1994) Morphological and biosystematics studies of Gelidium pusillum and G. pulchellum (Gelidiaceae, Rhodophyta) from Europe. Phycologia 33:462–470CrossRefGoogle Scholar
  10. Freshwater DW, Rueness J (1994) Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species, based on rbcL nucleotide sequence analysis. Phycologia 33:187–194CrossRefGoogle Scholar
  11. Freshwater DW, Tudor K, O’Shaughnessy K, Wysor B (2010) DNA barcoding in the red algal order Gelidiales: comparison of COI with rbcL and verification of the “barcoding gap”. Cryptogam Algol 31:435–449Google Scholar
  12. Geraldino PJL, Yang EC, Boo SM (2006) Morphology and molecular phylogeny of Hypnea flexicaulis (Gigartinales, Rhodophyta) from Korea. Algae 21:417–423CrossRefGoogle Scholar
  13. Geraldino PJL, Riosmena-Rodriguez R, Liao LM, Boo SM (2010) Phylogenetic relationships within the genus Hypnea (Gigartinales, Rhodophyta), with a description of H. caespitosa sp. nov. J Phycol 46:336–345CrossRefGoogle Scholar
  14. Guiry MD in Guiry MD, Guiry GM (2018) AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Available from: http://www.algaebase.org/. Accessed: 18 July 2018
  15. Hall TA (1999) BioEdit: a user-friendly biological alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  16. Iha C (2014) Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para a região Sudeste do Brasil. Dissertation, University of São PauloGoogle Scholar
  17. Iha C, Milstein D, Guimarães SMPB, Freshwater DW, Oliveira MC (2015) DNA barcoding reveals high diversity in the Gelidiales of the Brazilian southeast coast. Bot Mar 58:295–305CrossRefGoogle Scholar
  18. Iha C, O’Shaughnessy KA, Guimarães SMPB, Oliveira MC, Freshwater DW (2016) Taxonomic reappraisal of Gelidium coarctatum (Gelidiales, Rhodophyta) and Gelidium lineare sp. nov. from the tropical westrn Atlantic. Phycologia 55:555–563CrossRefGoogle Scholar
  19. Jamas M (2015) Diversidade de Gelidiales (Rhodophyta) no Brasil, com ênfase na região nordeste, com base em estudos morfológicos e DNA barcodes. Masters Thesis, Instituto de BotânicaGoogle Scholar
  20. Jamas M, Iha C, Oliveira MC, Guimarães SMPB, Fujii MT (2017) Morphological and molecular studies on Gelidiaceae and Gelidiellaceae (Gelidiales, Rhodophyta) from Brazil with description of the new species Gelidium calidum. Phytotaxa 314:195–218CrossRefGoogle Scholar
  21. Joly AB (1957) Contribuição ao conhecimento da flora ficológica marinha da baía de Santos e arredores. Bol da Fac Filos Ciências e Let Univ São Paulo, série Botânica 14: 1–196Google Scholar
  22. Kim KM, Boo SM (2012) Phylogenetic relationships and distribution of Gelidium crinale and G. pusillum (Gelidiales, Rhodophyta) using cox1 and rbcL sequences. Algae 27:83–94CrossRefGoogle Scholar
  23. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  24. Le Gall L, Saunders GW (2010) DNA barcoding is a powerful tool to uncover algal diversity: a case study of the Phyllophoraceae (Gigartinales, Rhodophyta) in the Canadian flora. J Phycol 46:374–389CrossRefGoogle Scholar
  25. Lindstrom SC, Fredericq S (2003) rbcL gene sequences reveal relationships among north-east Pacific species of Porphyra (Bangiales, Rhodophyta) and a new species, P. aestivalis. Phycol Res 51:211–224CrossRefGoogle Scholar
  26. McHugh DJ (1991) Worldwide distribution of commercial resources of seaweeds including Gelidium. Hydrobiologia 221:19–29CrossRefGoogle Scholar
  27. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefGoogle Scholar
  28. Saunders GW (2005) Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Phil Trans R Soc Lond B 360:1879–1888CrossRefGoogle Scholar
  29. Seo YB, Lee YW, Lee CH, You HC (2010) Red algae and their use in papermaking. Bioresour Technol 101:2549–2553CrossRefGoogle Scholar
  30. Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313CrossRefGoogle Scholar
  31. Thomas DT, Freshwater DW (2001) Studies of Costa Rican Gelidiales (Rhodophyta): four Caribbean taxa including Pterocladiella beachii sp. nov. Phycologia 40:340–350CrossRefGoogle Scholar
  32. Ugadim Y (1974) Algas Marinhas Bentônicas do Litoral Sul do Estado de São Paulo e do Litoral do estado do Paraná. III - Divisão Rhodophyta (1) Goniotricales, Bangiales, Nemaniolales e Gelidiales. Bol Botânica, Univ São Paulo 2:93–137Google Scholar
  33. Wynne MJ, Freshwater DW (2004) Gelidium omanense sp. nov. (Gelidiaceae, Rhodophyta) from Sultanate of Oman. Bot Mar 47:64–72CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Post-graduate Programme “Biodiversidade Vegetal e Meio Ambiente”Instituto de BotânicaSão PauloBrazil
  2. 2.Departamento de Ciências do MarUniversidade Federal de São PauloSantosBrazil
  3. 3.Department of BiologyChungnam National UniversityDaejeonSouth Korea
  4. 4.Núcleo de Pesquisa em Ficologia, Instituto de BotânicaSão PauloBrazil

Personalised recommendations