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Journal of Applied Phycology

, Volume 30, Issue 6, pp 3299–3310 | Cite as

Effect of plant growth regulators on direct regeneration and callus induction from Sargassum polycystum C. Agardh

  • Siti Nor Safriena Muhamad
  • Anna Pick-Kiong Ling
  • Ching-Lee WongEmail author
8th Asian Pacific Phycological Forum

Abstract

Seaweed tissue culture is one of the potential technologies that can be used to increase seaweed production to cope with increasing demand. Seaweed tissue culture can be divided into two methods, namely, direct regeneration and callus induction (indirect regeneration). These methods provide several advantages, including generating seaweed cultures that are disease-free and able to mature faster to support mass production. The present study aims to develop a suitable protocol for tissue culture of Sargassum polycystum C. Agardh. We investigated different methods of sterilizing the stipe, stolon and leaf explant surfaces to allow for axenic tissue growth. In addition, we also investigated the effects of different plant growth regulators (PGRs) on seaweeds that are grown via direct regeneration and callus induction method. Our results showed that a combination of physical (brushing using soft brush under microscope) and chemical treatments (detergent, povidone iodine (PI) and antibiotic solution of streptomycin sulphate) was most suitable for growing axenic stipe of S. polycystum. For obtaining axenic leaf and stolon explants, the best treatment option was using germanium oxide (GeO2) as a pre-treatment, followed by treatments with detergent, PI and streptomycin sulphate antibiotic solution. We tested the effects of nine different plant growth regulators on the growth of tissue explants, which include indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-napthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), picloram, forchlorfenuron (CPPU), uniconazole, 6-benzylaminopurine (BAP) and kinetin. Results showed that only bud explant was able to induce callus growth when treated with kinetin (4.65 μM and 13.94 μM) and uniconazole (3.00 μM, 7.00 μM and 10.00 μM). In addition, only stipe explant showed regeneration of adventitious bud when grown in the absence of plant growth regulators.

Keywords

Sargassum polycystum Phaeophyceae Adventitious bud regeneration Callus induction Plant growth regulator Seaweed tissue culture 

Notes

Funding information

The authors would like to thank the funding and technical supports from the Ministry of Education Malaysia (FRGS/1/2013/STWN03/TAYLOR/02/1) and Taylor’s University.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Siti Nor Safriena Muhamad
    • 1
  • Anna Pick-Kiong Ling
    • 2
  • Ching-Lee Wong
    • 1
    Email author
  1. 1.School of BiosciencesTaylor’s University, Taylor’s Lakeside CampusSubang JayaMalaysia
  2. 2.Division of Applied Biomedical Sciences and Biotechnology, School of Health SciencesInternational Medical UniversityKuala LumpurMalaysia

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